中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro

文献类型:期刊论文

作者Wang, Li1,2,3; An, Lizhe2; Hu, Yanping1; Wei, Lixin1; Li, Yi1
刊名african journal of biotechnology
出版日期2009-06-03
卷号8期号:11页码:2513-2517
关键词Swertia chirata adventitious shoots histology an orthogonal test leaves
ISSN号1684-5315
中文摘要a procedure for in vitro shoot regeneration was formed from leaves derived from the field grown swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. the best result was obtained in the 190-2 medium with 13.32 mu m 6-benzylaminopurine and 0.54 mu m alpha-naphthaleneacetic acid. the result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. the propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. the highest rate of shoots was 96.50% on the medium with 5.40 mu m alpha-naphthaleneacetic acid. histological studies showed shoot regeneration took place by somatic embryogenesis.
英文摘要a procedure for in vitro shoot regeneration was formed from leaves derived from the field grown swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. the best result was obtained in the 190-2 medium with 13.32 mu m 6-benzylaminopurine and 0.54 mu m alpha-naphthaleneacetic acid. the result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. the propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. the highest rate of shoots was 96.50% on the medium with 5.40 mu m alpha-naphthaleneacetic acid. histological studies showed shoot regeneration took place by somatic embryogenesis.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biotechnology & applied microbiology
研究领域[WOS]biotechnology & applied microbiology
关键词[WOS]endangered medicinal herb ; somatic embryogenesis ; explants ; multiplication ; organogenesis ; cultures ; cassava ; plants
收录类别SCI
语种英语
WOS记录号WOS:000268830900018
公开日期2011-12-13
源URL[http://ir.nwipb.ac.cn//handle/363003/1858]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Wang, Li,An, Lizhe,Hu, Yanping,et al. Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro[J]. african journal of biotechnology,2009,8(11):2513-2517.
APA Wang, Li,An, Lizhe,Hu, Yanping,Wei, Lixin,&Li, Yi.(2009).Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro.african journal of biotechnology,8(11),2513-2517.
MLA Wang, Li,et al."Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro".african journal of biotechnology 8.11(2009):2513-2517.

入库方式: OAI收割

来源:西北高原生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。