Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro
文献类型：期刊论文
| 作者 | Wang, Li1,2,3; An, Lizhe2; Hu, Yanping1; Wei, Lixin1; Li, Yi1 |
| 刊名 | african journal of biotechnology
 |
| 出版日期 | 2009-06-03 |
| 卷号 | 8期号:11页码:2513-2517 |
| 关键词 | Swertia chirata adventitious shoots histology an orthogonal test leaves |
| ISSN号 | 1684-5315 |
| 中文摘要 | a procedure for in vitro shoot regeneration was formed from leaves derived from the field grown swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. the best result was obtained in the 190-2 medium with 13.32 mu m 6-benzylaminopurine and 0.54 mu m alpha-naphthaleneacetic acid. the result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. the propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. the highest rate of shoots was 96.50% on the medium with 5.40 mu m alpha-naphthaleneacetic acid. histological studies showed shoot regeneration took place by somatic embryogenesis. |
| 英文摘要 | a procedure for in vitro shoot regeneration was formed from leaves derived from the field grown swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. the best result was obtained in the 190-2 medium with 13.32 mu m 6-benzylaminopurine and 0.54 mu m alpha-naphthaleneacetic acid. the result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. the propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. the highest rate of shoots was 96.50% on the medium with 5.40 mu m alpha-naphthaleneacetic acid. histological studies showed shoot regeneration took place by somatic embryogenesis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | endangered medicinal herb ; somatic embryogenesis ; explants ; multiplication ; organogenesis ; cultures ; cassava ; plants |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268830900018 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1858]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Li,An, Lizhe,Hu, Yanping,et al. Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro[J]. african journal of biotechnology,2009,8(11):2513-2517. |
| APA | Wang, Li,An, Lizhe,Hu, Yanping,Wei, Lixin,&Li, Yi.(2009).Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro.african journal of biotechnology,8(11),2513-2517. |
| MLA | Wang, Li,et al."Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro".african journal of biotechnology 8.11(2009):2513-2517. |
入库方式： OAI收割
来源：西北高原生物研究所
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