A sensitive fluorescence reagent, 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source
文献类型：期刊论文
| 作者 | Li, Guoliang1,2; Sun, Zhiwei1,2; Song, Cuihua3; Xia, Lian1,2; Zheng, Jie1,2; Suo, Yourui1; You, Jinmao1,3 |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 25期号:6页码:689-696 |
| 关键词 | amino acids analysis HPLC-FLD-MS/ESI pre-column derivatization Tibetan medicine Saussurea involucrate Artemisia capillaris Thunb |
| ISSN号 | 0269-3879 |
| 中文摘要 | recent researches shows that amino acids (aa) are not only cell signaling molecules but are also regulators of gene expression and the protein phosphorylation cascade. more precise analysis of aa composition is reckoned to be one of the most important applications in the biomedical and pharmaceutical fields. in this paper, we develop a sample, sensitive and mild method using 2-[2-(7h-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate (dbcec) as a labeling reagent for aa determination by high-performance liquid chromatography (hplc) with fluorescence detection (fld) and identification with mass spectroscopy. the maximum excitation and emission wavelengths for dbcec-aa derivatives were 300 and 395 nm, respectively. this method, in conjunction with a gradient elution, offered a baseline resolution of 20 aa on a reversed-phase hypersil bds c(18) column. lc separation for the derivatized aa showed good reproducibility, and all aa were found to give excellent linear responses with correlation coefficients > 0.9993. the calculated detection limits with a 25.0 fmol injection of each aa (at a signal-to-noise ratio of 3:1) ranged from 2.62 to 22.6 fmol. this method was applied to determine the aa composition in saussurea involucrate and artemisia capillaris thunb. meanwhile, this method exhibits a powerful potential for trace analysis of aa from biomedicine, foodstuff and other complex samples. copyright (c) 2010 john wiley & sons, ltd. |
| 英文摘要 | recent researches shows that amino acids (aa) are not only cell signaling molecules but are also regulators of gene expression and the protein phosphorylation cascade. more precise analysis of aa composition is reckoned to be one of the most important applications in the biomedical and pharmaceutical fields. in this paper, we develop a sample, sensitive and mild method using 2-[2-(7h-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate (dbcec) as a labeling reagent for aa determination by high-performance liquid chromatography (hplc) with fluorescence detection (fld) and identification with mass spectroscopy. the maximum excitation and emission wavelengths for dbcec-aa derivatives were 300 and 395 nm, respectively. this method, in conjunction with a gradient elution, offered a baseline resolution of 20 aa on a reversed-phase hypersil bds c(18) column. lc separation for the derivatized aa showed good reproducibility, and all aa were found to give excellent linear responses with correlation coefficients > 0.9993. the calculated detection limits with a 25.0 fmol injection of each aa (at a signal-to-noise ratio of 3:1) ranged from 2.62 to 22.6 fmol. this method was applied to determine the aa composition in saussurea involucrate and artemisia capillaris thunb. meanwhile, this method exhibits a powerful potential for trace analysis of aa from biomedicine, foodstuff and other complex samples. copyright (c) 2010 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | 9-fluorenylmethyl chloroformate ; precolumn derivatization ; gas-chromatography ; phase ; chloride ; hplc ; spectrometry ; phthaldialdehyde ; supplementation ; constituents |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290480000009 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1540]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guoliang,Sun, Zhiwei,Song, Cuihua,et al. A sensitive fluorescence reagent, 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source[J]. biomedical chromatography,2011,25(6):689-696. |
| APA | Li, Guoliang.,Sun, Zhiwei.,Song, Cuihua.,Xia, Lian.,Zheng, Jie.,...&You, Jinmao.(2011).A sensitive fluorescence reagent, 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source.biomedical chromatography,25(6),689-696. |
| MLA | Li, Guoliang,et al."A sensitive fluorescence reagent, 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy]ethyl chloroformate, for amino acids determination in Saussurea involucrate and Artemisia capillaris Thunb using high-performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source".biomedical chromatography 25.6(2011):689-696. |
入库方式： OAI收割
来源：西北高原生物研究所
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