Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification
文献类型：期刊论文
| 作者 | Sun, Zhiwei2,4; You, Jinmao1,2; Li, Guoliang2,4; Zhao, Xian'en3; Suo, Yourui2; Wang, Xiao3 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2011-05-15 |
| 卷号 | 879期号:17-18页码:1367-1374 |
| 关键词 | Amino acid Derivatization 1,2,5,6-Dibenzocarbazole-9-ethyl chloroformate High performance liquid chromatography Fluorescence detection Electrospray mass spectrometer |
| ISSN号 | 1570-0232 |
| 中文摘要 | a high performance liquid chromatography method for the determination of 20 amino acids (aas), using 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate (dbceoc-ci) as a novel fluorescent labeling reagent, has been developed and applied for the analysis of aas in rat brain microdialysate. the simultaneous separation of 20 aa derivatives was achieved on a hypersil bds c(18) column with gradient elution. and the identification of aa derivatives was carried out by on-line electrospray ionization mass spectrometry in positive ion mode. the aa derivatives were detected with excitation and emission at 300 nm and 395 nm, respectively. excellent linear responses were observed with coefficients of >0.9988. the detection limits ranged from 0.217 to 4.75 nmol/l, at a signal-to-noise ratio of 3. the intra-day and inter-day precision for each aa was <3.2% and <4.3%. respectively. the mean recoveries for all aas studied were in the range of 92.5-105.6%. good compositional data could be obtained from as little as 15 mu l of microdialysate. facile dbceoc-ci derivatization coupled with high performance liquid chromatography and fluorescence detection allowed the development of a highly sensitive method for the quantitative analysis of trace levels of aas from microdialysate. (c) 2011 elsevier b.v. all rights reserved. |
| 英文摘要 | a high performance liquid chromatography method for the determination of 20 amino acids (aas), using 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate (dbceoc-ci) as a novel fluorescent labeling reagent, has been developed and applied for the analysis of aas in rat brain microdialysate. the simultaneous separation of 20 aa derivatives was achieved on a hypersil bds c(18) column with gradient elution. and the identification of aa derivatives was carried out by on-line electrospray ionization mass spectrometry in positive ion mode. the aa derivatives were detected with excitation and emission at 300 nm and 395 nm, respectively. excellent linear responses were observed with coefficients of >0.9988. the detection limits ranged from 0.217 to 4.75 nmol/l, at a signal-to-noise ratio of 3. the intra-day and inter-day precision for each aa was <3.2% and <4.3%. respectively. the mean recoveries for all aas studied were in the range of 92.5-105.6%. good compositional data could be obtained from as little as 15 mu l of microdialysate. facile dbceoc-ci derivatization coupled with high performance liquid chromatography and fluorescence detection allowed the development of a highly sensitive method for the quantitative analysis of trace levels of aas from microdialysate. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | precolumn derivatization ; biogenic-amines ; capillary-electrophoresis ; inferior colliculus ; chloride ; quantitation ; salicylate ; polyamines ; peptides ; matrix |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291131900019 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1546]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 3.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,You, Jinmao,Li, Guoliang,et al. Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(17-18):1367-1374. |
| APA | Sun, Zhiwei,You, Jinmao,Li, Guoliang,Zhao, Xian'en,Suo, Yourui,&Wang, Xiao.(2011).Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(17-18),1367-1374. |
| MLA | Sun, Zhiwei,et al."Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.17-18(2011):1367-1374. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。