Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits
文献类型：期刊论文
| 作者 | Li, Guo-Liang1,3; You, Jin-Mao1,2; Song, Cui-Hua2; Xia, Lian2; Zheng, Jie1,3; Suo, You-Rui1 |
| 刊名 | journal of agricultural and food chemistry
 |
| 出版日期 | 2011-04-13 |
| 卷号 | 59期号:7页码:2972-2979 |
| 关键词 | triterpenic acids HPLC-FLD precolumn fluorescent derivatization fruits |
| ISSN号 | 0021-8561 |
| 中文摘要 | triterpenic acids are widespread in plants and have multiplicity of biological properties. unfortunately the method for accurate analysis of these compounds remains poorly investigated. this study proposed a highly sensitive and selective precolumn derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (aets) as fluorescent labeling reagent by hplc with fluorescence detection (fld). response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labeling of the analyzed components. the rapid separation of five triterpenic acids could be achieved in as little as 16 mm. this developed method offered the exciting detection limits of 1.68-2.04 ng/ml. when applied to several popular fruits in china, it revealed satisfactory applicability and reproducibility. this developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products. |
| 英文摘要 | triterpenic acids are widespread in plants and have multiplicity of biological properties. unfortunately the method for accurate analysis of these compounds remains poorly investigated. this study proposed a highly sensitive and selective precolumn derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (aets) as fluorescent labeling reagent by hplc with fluorescence detection (fld). response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labeling of the analyzed components. the rapid separation of five triterpenic acids could be achieved in as little as 16 mm. this developed method offered the exciting detection limits of 1.68-2.04 ng/ml. when applied to several popular fruits in china, it revealed satisfactory applicability and reproducibility. this developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | agriculture, multidisciplinary ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | agriculture ; chemistry ; food science & technology |
| 关键词[WOS] | performance liquid-chromatography ; ursolic acid ; oleanolic acid ; fatty-acids ; optimization ; identification ; extracts ; cancer ; electrophoresis ; quantification |
| 收录类别 | SCI ; IC |
| 语种 | 英语 |
| WOS记录号 | WOS:000289050400031 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1558]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guo-Liang,You, Jin-Mao,Song, Cui-Hua,et al. Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits[J]. journal of agricultural and food chemistry,2011,59(7):2972-2979. |
| APA | Li, Guo-Liang,You, Jin-Mao,Song, Cui-Hua,Xia, Lian,Zheng, Jie,&Suo, You-Rui.(2011).Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits.journal of agricultural and food chemistry,59(7),2972-2979. |
| MLA | Li, Guo-Liang,et al."Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits".journal of agricultural and food chemistry 59.7(2011):2972-2979. |
入库方式： OAI收割
来源：西北高原生物研究所
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