Determination of trace amino acids in human serum by a selective and sensitive pre-column derivatization method using HPLC-FLD-MS/MS and derivatization optimization by response surface methodology
文献类型：期刊论文
| 作者 | Li, Guoliang2,3; Cui, Yanyan5; You, Jinmao1,3; Zhao, Xianen4; Sun, Zhiwei2,3; Xia, Lian1,2,3; Suo, Yourui3; Wang, Xiao4 |
| 刊名 | amino acids
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 40期号:4页码:1185-1193 |
| 关键词 | Trace amino acids analysis Human serum HPLC-FLD-MS/MS Pre-column derivatization Response surface methodology |
| ISSN号 | 0939-4451 |
| 中文摘要 | analysis of trace amino acids (aa) in physiological fluids has received more attention, because the analysis of these compounds could provide fundamental and important information for medical, biological, and clinical researches. more accurate method for the determination of those compounds is highly desirable and valuable. in the present study, we developed a selective and sensitive method for trace aa determination in biological samples using 2-[2-(7h-dibenzo [a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (dbcec) as labeling reagent by hplc-fld-ms/ms. response surface methodology (rsm) was first employed to optimize the derivatization reaction between dbcec and aa. compared with traditional single-factor design, rsm was capable of lessening laborious, time and reagents consumption. the complete derivatization can be achieved within 6.3 min at room temperature. in conjunction with a gradient elution, a baseline resolution of 20 aa containing acidic, neutral, and basic aa was achieved on a reversed-phase hypersil bds c(18) column. this method showed excellent reproducibility and correlation coefficient, and offered the exciting detection limits of 0.19-1.17 fmol/mu l. the developed method was successfully applied to determinate aa in human serum. the sensitive and prognostic index of serum aa for liver diseases has also been discussed. |
| 英文摘要 | analysis of trace amino acids (aa) in physiological fluids has received more attention, because the analysis of these compounds could provide fundamental and important information for medical, biological, and clinical researches. more accurate method for the determination of those compounds is highly desirable and valuable. in the present study, we developed a selective and sensitive method for trace aa determination in biological samples using 2-[2-(7h-dibenzo [a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (dbcec) as labeling reagent by hplc-fld-ms/ms. response surface methodology (rsm) was first employed to optimize the derivatization reaction between dbcec and aa. compared with traditional single-factor design, rsm was capable of lessening laborious, time and reagents consumption. the complete derivatization can be achieved within 6.3 min at room temperature. in conjunction with a gradient elution, a baseline resolution of 20 aa containing acidic, neutral, and basic aa was achieved on a reversed-phase hypersil bds c(18) column. this method showed excellent reproducibility and correlation coefficient, and offered the exciting detection limits of 0.19-1.17 fmol/mu l. the developed method was successfully applied to determinate aa in human serum. the sensitive and prognostic index of serum aa for liver diseases has also been discussed. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | performance liquid-chromatography ; fluorescence detection ; chronic hepatitis ; human plasma ; disease ; chloroformate ; reagent ; brain |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288546700015 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1564]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Jining 273165, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 4.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China 5.88 Mil Hosp China, Tai An 271000, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guoliang,Cui, Yanyan,You, Jinmao,et al. Determination of trace amino acids in human serum by a selective and sensitive pre-column derivatization method using HPLC-FLD-MS/MS and derivatization optimization by response surface methodology[J]. amino acids,2011,40(4):1185-1193. |
| APA | Li, Guoliang.,Cui, Yanyan.,You, Jinmao.,Zhao, Xianen.,Sun, Zhiwei.,...&Wang, Xiao.(2011).Determination of trace amino acids in human serum by a selective and sensitive pre-column derivatization method using HPLC-FLD-MS/MS and derivatization optimization by response surface methodology.amino acids,40(4),1185-1193. |
| MLA | Li, Guoliang,et al."Determination of trace amino acids in human serum by a selective and sensitive pre-column derivatization method using HPLC-FLD-MS/MS and derivatization optimization by response surface methodology".amino acids 40.4(2011):1185-1193. |
入库方式： OAI收割
来源：西北高原生物研究所
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