Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology
文献类型：期刊论文
| 作者 | Li, Guoliang1,3; Zhang, Xiaolong3,4; You, Jinmao1,2; Song, Cuihua2; Sun, Zhiwei1,2; Xia, Lian1,2; Suo, Yourui1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2011-03-04 |
| 卷号 | 688期号:2页码:208-218 |
| 关键词 | Oleanolic acids Ursolic acids Pre-column derivatization HPLC-FLD Swertia species Response surface methodology |
| ISSN号 | 0003-2670 |
| 中文摘要 | oleanolic acid (oa) and ursolic acid (ua) are the ubiquitous triterpenic acids in plant kingdom and have multiple biological effects. in the present study, a highly sensitive and selective method using a dual-sensitive probe 2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets) as pre-column labeling reagent has been developed for rapid determination of the triterpenes oa and ua by hplc with fluorescence detection (fld) and online mass spectrometry identification. response surface methodology as an efficient tool was employed to optimize the ultrasonic-assisted extraction of triterpenic acids from swertia plants and the pre-column derivatization reaction, respectively, which ensured the highest triterpenic acids recoveries within the shortest extraction time and the sufficient labeling of the analyzed components. fast separation of the isomers oa and ua could be achieved on a hypersil bds c8 column within 7 min. both of oa and ua gave the good correlation coefficients of 0.9999. this developed method offered the satisfactory detection limits of 1.10 and 1.30 ng ml(-1) for ua and oa, respectively. when applied to swertia species, it showed good reproducibility. (c) 2011 elsevier b.v. all rights reserved. |
| 英文摘要 | oleanolic acid (oa) and ursolic acid (ua) are the ubiquitous triterpenic acids in plant kingdom and have multiple biological effects. in the present study, a highly sensitive and selective method using a dual-sensitive probe 2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets) as pre-column labeling reagent has been developed for rapid determination of the triterpenes oa and ua by hplc with fluorescence detection (fld) and online mass spectrometry identification. response surface methodology as an efficient tool was employed to optimize the ultrasonic-assisted extraction of triterpenic acids from swertia plants and the pre-column derivatization reaction, respectively, which ensured the highest triterpenic acids recoveries within the shortest extraction time and the sufficient labeling of the analyzed components. fast separation of the isomers oa and ua could be achieved on a hypersil bds c8 column within 7 min. both of oa and ua gave the good correlation coefficients of 0.9999. this developed method offered the satisfactory detection limits of 1.10 and 1.30 ng ml(-1) for ua and oa, respectively. when applied to swertia species, it showed good reproducibility. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | fluorescence detection |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288468000014 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1576]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Chendu Inst Biol, Chengdu 610041, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guoliang,Zhang, Xiaolong,You, Jinmao,et al. Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology[J]. analytica chimica acta,2011,688(2):208-218. |
| APA | Li, Guoliang.,Zhang, Xiaolong.,You, Jinmao.,Song, Cuihua.,Sun, Zhiwei.,...&Suo, Yourui.(2011).Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology.analytica chimica acta,688(2),208-218. |
| MLA | Li, Guoliang,et al."Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology".analytica chimica acta 688.2(2011):208-218. |
入库方式： OAI收割
来源：西北高原生物研究所
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