中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology

文献类型:期刊论文

作者Li, Guoliang1,3; Zhang, Xiaolong3,4; You, Jinmao1,2; Song, Cuihua2; Sun, Zhiwei1,2; Xia, Lian1,2; Suo, Yourui1
刊名analytica chimica acta
出版日期2011-03-04
卷号688期号:2页码:208-218
关键词Oleanolic acids Ursolic acids Pre-column derivatization HPLC-FLD Swertia species Response surface methodology
ISSN号0003-2670
中文摘要oleanolic acid (oa) and ursolic acid (ua) are the ubiquitous triterpenic acids in plant kingdom and have multiple biological effects. in the present study, a highly sensitive and selective method using a dual-sensitive probe 2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets) as pre-column labeling reagent has been developed for rapid determination of the triterpenes oa and ua by hplc with fluorescence detection (fld) and online mass spectrometry identification. response surface methodology as an efficient tool was employed to optimize the ultrasonic-assisted extraction of triterpenic acids from swertia plants and the pre-column derivatization reaction, respectively, which ensured the highest triterpenic acids recoveries within the shortest extraction time and the sufficient labeling of the analyzed components. fast separation of the isomers oa and ua could be achieved on a hypersil bds c8 column within 7 min. both of oa and ua gave the good correlation coefficients of 0.9999. this developed method offered the satisfactory detection limits of 1.10 and 1.30 ng ml(-1) for ua and oa, respectively. when applied to swertia species, it showed good reproducibility. (c) 2011 elsevier b.v. all rights reserved.
英文摘要oleanolic acid (oa) and ursolic acid (ua) are the ubiquitous triterpenic acids in plant kingdom and have multiple biological effects. in the present study, a highly sensitive and selective method using a dual-sensitive probe 2-(5-benzoacridine)ethyl-p-toluenesulfonate (baets) as pre-column labeling reagent has been developed for rapid determination of the triterpenes oa and ua by hplc with fluorescence detection (fld) and online mass spectrometry identification. response surface methodology as an efficient tool was employed to optimize the ultrasonic-assisted extraction of triterpenic acids from swertia plants and the pre-column derivatization reaction, respectively, which ensured the highest triterpenic acids recoveries within the shortest extraction time and the sufficient labeling of the analyzed components. fast separation of the isomers oa and ua could be achieved on a hypersil bds c8 column within 7 min. both of oa and ua gave the good correlation coefficients of 0.9999. this developed method offered the satisfactory detection limits of 1.10 and 1.30 ng ml(-1) for ua and oa, respectively. when applied to swertia species, it showed good reproducibility. (c) 2011 elsevier b.v. all rights reserved.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, analytical
研究领域[WOS]chemistry
关键词[WOS]fluorescence detection
收录类别SCI
语种英语
WOS记录号WOS:000288468000014
公开日期2011-12-13
源URL[http://ir.nwipb.ac.cn//handle/363003/1576]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Chinese Acad Sci, Chendu Inst Biol, Chengdu 610041, Peoples R China
推荐引用方式
GB/T 7714
Li, Guoliang,Zhang, Xiaolong,You, Jinmao,et al. Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology[J]. analytica chimica acta,2011,688(2):208-218.
APA Li, Guoliang.,Zhang, Xiaolong.,You, Jinmao.,Song, Cuihua.,Sun, Zhiwei.,...&Suo, Yourui.(2011).Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology.analytica chimica acta,688(2),208-218.
MLA Li, Guoliang,et al."Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: Optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology".analytica chimica acta 688.2(2011):208-218.

入库方式: OAI收割

来源:西北高原生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。