LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification
文献类型：期刊论文
| 作者 | Kang, Li1; You, Jinmao1,2; Sun, Zhiwei1,3; Wang, Chengyan2; Ji, Zhongyin2; Gao, Yongping2; Suo, Yourui1; Li, Yulin1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2011 |
| 卷号 | 73期号:1-2页码:43-50 |
| 关键词 | Column liquid chromatography Pre-column derivatization Fluorescence detection Mass spectrometry Biogenic amines |
| ISSN号 | 0009-5893 |
| 中文摘要 | a pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (easc) as labeling reagent with fluorescence detection and mass spectrometry (ms) identification has been developed. after pre-column derivatization, the labeled biogenic amines were separated on a hypersil bds-c18 column by gradient elution. the derivatives showed an intense protonated molecular ion corresponding m/z [m + h](+) in positive-ion mode. the collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol l(-1) with coefficients of > 0.9993. detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). the relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. recoveries except for put were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage the established method for the determination of biogenic amines except for put from real samples was satisfactory. |
| 英文摘要 | a pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (easc) as labeling reagent with fluorescence detection and mass spectrometry (ms) identification has been developed. after pre-column derivatization, the labeled biogenic amines were separated on a hypersil bds-c18 column by gradient elution. the derivatives showed an intense protonated molecular ion corresponding m/z [m + h](+) in positive-ion mode. the collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol l-1 with coefficients of > 0.9993. detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). the relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. recoveries except for put were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage the established method for the determination of biogenic amines except for put from real samples was satisfactory. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | liquid-chromatographic determination ; ionization mass-spectrometry ; capillary-electrophoresis ; precolumn derivatization ; dansyl derivatives ; performance ; wine ; quantification ; extraction ; cadaverine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286670500007 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1604]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, Li,You, Jinmao,Sun, Zhiwei,et al. LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification[J]. chromatographia,2011,73(1-2):43-50. |
| APA | Kang, Li.,You, Jinmao.,Sun, Zhiwei.,Wang, Chengyan.,Ji, Zhongyin.,...&Li, Yulin.(2011).LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification.chromatographia,73(1-2),43-50. |
| MLA | Kang, Li,et al."LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification".chromatographia 73.1-2(2011):43-50. |
入库方式： OAI收割
来源：西北高原生物研究所
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