LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate as Labeling Reagent
文献类型：期刊论文
| 作者 | Sun, Zhiwei1,3; You, Jinmao1,2; Song, Cuihua2 |
| 刊名 | chromatographia
 |
| 出版日期 | 2010-10-01 |
| 卷号 | 72期号:7-8页码:641-649 |
| 关键词 | Column liquid chromatography Fluorescence detection Electrospray mass spectrometry Amino acids Stellera chamaejasme L. |
| ISSN号 | 0009-5893 |
| 中文摘要 | a pre-column derivatization method for the sensitive determination of amino acids using the tagging reagent 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (dbcec) followed by liquid chromatography with fluorescence detection has been developed. identification of dbcec-amino acids derivatives was by liquid chromatography-electrospray ionization mass spectrometry (lc-esi-ms-ms). dbcec can easily and quickly label amino acids, and derivatives are stable enough to be efficiently analyzed by lc. separation of the derivatized amino acids had been optimized on hypersil bds c(18) column. a perfect baseline separation for 20 amino acid derivatives was achieved with a ternary gradient elution program. the chromophore of dibenzocarbazole group, which comprise a large rigid planar structure with p-pi conjugation system, resulted in a sensitive fluorescence detection for amino acid derivatives. the derivatized amino acids were detected with fluorescence detector with excitation maximum and emission maximum at 300 and 390 nm, respectively. excellent linear responses were observed with coefficients of > 0.9993, and detection limits were in the range of 0.78-5.13 fmol (signal-to-noise ratio of 3). the mean accuracy ranged from 83.4 to 98.7% for fluorescence detection. the mean inter-day precision for all standards was < 4.2% of the expected concentration. therefore, the proposed method was a highly sensitive and specific method for the quantitative analysis of amino acids from biological and natural environmental samples. |
| 英文摘要 | a pre-column derivatization method for the sensitive determination of amino acids using the tagging reagent 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (dbcec) followed by liquid chromatography with fluorescence detection has been developed. identification of dbcec-amino acids derivatives was by liquid chromatography-electrospray ionization mass spectrometry (lc-esi-ms-ms). dbcec can easily and quickly label amino acids, and derivatives are stable enough to be efficiently analyzed by lc. separation of the derivatized amino acids had been optimized on hypersil bds c(18) column. a perfect baseline separation for 20 amino acid derivatives was achieved with a ternary gradient elution program. the chromophore of dibenzocarbazole group, which comprise a large rigid planar structure with p-pi conjugation system, resulted in a sensitive fluorescence detection for amino acid derivatives. the derivatized amino acids were detected with fluorescence detector with excitation maximum and emission maximum at 300 and 390 nm, respectively. excellent linear responses were observed with coefficients of > 0.9993, and detection limits were in the range of 0.78-5.13 fmol (signal-to-noise ratio of 3). the mean accuracy ranged from 83.4 to 98.7% for fluorescence detection. the mean inter-day precision for all standards was < 4.2% of the expected concentration. therefore, the proposed method was a highly sensitive and specific method for the quantitative analysis of amino acids from biological and natural environmental samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; ionization-mass-spectrometry ; capillary-electrophoresis ; biogenic-amines ; precolumn derivatization ; identification ; hplc ; derivatives ; separation ; carbamate |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000282211200010 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1648]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,You, Jinmao,Song, Cuihua. LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate as Labeling Reagent[J]. chromatographia,2010,72(7-8):641-649. |
| APA | Sun, Zhiwei,You, Jinmao,&Song, Cuihua.(2010).LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate as Labeling Reagent.chromatographia,72(7-8),641-649. |
| MLA | Sun, Zhiwei,et al."LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using 2-[2-(Dibenzocarbazole)-ethoxy] Ethyl Chloroformate as Labeling Reagent".chromatographia 72.7-8(2010):641-649. |
入库方式： OAI收割
来源：西北高原生物研究所
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