A potato NOA gene increased salinity tolerance in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Zhang, B.1,2; Wang, H. Q.1; Liu, B. L.1; Liu, J.1,2; Wang, X.1,2; Liu, Q.1,2; Zhang, H. G.1 |
| 刊名 | african journal of biotechnology
 |
| 出版日期 | 2010-09-06 |
| 卷号 | 9期号:36页码:5869-5878 |
| 关键词 | StNOA1 transformation Solanum tuberosum Atnoa1 mutant salt tolerance |
| ISSN号 | 1684-5315 |
| 中文摘要 | the purpose of this study is to produce recombinant stnoa1 in transgenic plants and to test its potential role in plant salt stress responses. the newly cloned stnoa1 gene from solanum tuberosum l. was inserted into atnoa1 mutant plant genome by agrobaterium-mediated floral dip method. transgene integration was verified by polymerase chain reaction (pcr) in 4 different lines of transgenic atnoa1. expression of stnoa1 gene was further analyzed by reverse trancription (rt)-pcr. physiological analyses indicated that the transgenic line tl9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (mda) compared to its receptor, atnoa1 mutant, under salt stress condition. root elongation and survival rate in tl9 were significantly higher than those in atnoa1 seedlings under salt stress. present study proved that stnoa1 participated in arabidopsis thaliana salt stress responses and increased its salinity tolerance. |
| 英文摘要 | the purpose of this study is to produce recombinant stnoa1 in transgenic plants and to test its potential role in plant salt stress responses. the newly cloned stnoa1 gene from solanum tuberosum l. was inserted into atnoa1 mutant plant genome by agrobaterium-mediated floral dip method. transgene integration was verified by polymerase chain reaction (pcr) in 4 different lines of transgenic atnoa1. expression of stnoa1 gene was further analyzed by reverse trancription (rt)-pcr. physiological analyses indicated that the transgenic line tl9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (mda) compared to its receptor, atnoa1 mutant, under salt stress condition. root elongation and survival rate in tl9 were significantly higher than those in atnoa1 seedlings under salt stress. present study proved that stnoa1 participated in arabidopsis thaliana salt stress responses and increased its salinity tolerance. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | nitric-oxide synthase ; nitrate reductase ; stomatal closure ; oxidative stress ; old enzyme ; 2 ecotypes ; plants ; identification ; responses ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281680400014 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1654]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, B.,Wang, H. Q.,Liu, B. L.,et al. A potato NOA gene increased salinity tolerance in Arabidopsis thaliana[J]. african journal of biotechnology,2010,9(36):5869-5878. |
| APA | Zhang, B..,Wang, H. Q..,Liu, B. L..,Liu, J..,Wang, X..,...&Zhang, H. G..(2010).A potato NOA gene increased salinity tolerance in Arabidopsis thaliana.african journal of biotechnology,9(36),5869-5878. |
| MLA | Zhang, B.,et al."A potato NOA gene increased salinity tolerance in Arabidopsis thaliana".african journal of biotechnology 9.36(2010):5869-5878. |
入库方式： OAI收割
来源：西北高原生物研究所
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