Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification
文献类型：期刊论文
| 作者 | Li Xiao-Yan1; You Jin-Mao1,2; Sun Zhi-Wei1,3; Fu Yan-Yan1; Qin Xue-Qin1; Ding Ming-Zhou1; Li Yu-Lin2 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 38期号:7页码:917-923 |
| 关键词 | High performance liquid chromatography-ion trap mass spectrometry Fluorescence detection Pre-column derivatization 2-[2-(7H-Dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate Amino acids |
| ISSN号 | 0253-3820 |
| 中文摘要 | by using 2-[2-(7h-dibenzo[a,g]carbazole-ethoxy)-ethyl chloroformate (dbcec-cl) as a sensitive pre-column derivatization reagent, a simple method for evaluating the chemical compositions of protein amino acids from the hydrolyzed cicada sample has been developed. studies on derivatization conditions indicated that the labeling reaction proceeded rapidly and smoothly in the presence of a base catalyst ( ph 9.0) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 300 nm and an emission maximum at lambda(em) 395 nm. the separation of amino acid derivatives was carried out on a reversed phase hypersil bds-c(18) (200 mm x 4. 6 mm x 5 mu m) column in combination of a gradient elution. the complete baseline resolution for 20 amino acid derivatives was achieved within 65 min. identification of amino acid derivatives was carried out by the online post-column ion trap/mass spectrometry with electrospray ion source (esi source) in positive ion mode. excellent linear response was observed, with coefficients of >0.9992. fluorescent detection limits calculated from 25 pmol injection, at a signal-to-noise ratio of 3, were 2.6 - 24 fmol. the established method for the determination of amino acids from cicada sample was satisfactory. |
| 英文摘要 | by using 2-[2-(7h-dibenzo[a,g]carbazole-ethoxy)-ethyl chloroformate (dbcec-cl) as a sensitive pre-column derivatization reagent, a simple method for evaluating the chemical compositions of protein amino acids from the hydrolyzed cicada sample has been developed. studies on derivatization conditions indicated that the labeling reaction proceeded rapidly and smoothly in the presence of a base catalyst ( ph 9.0) in acetonitrile to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) 300 nm and an emission maximum at lambda(em) 395 nm. the separation of amino acid derivatives was carried out on a reversed phase hypersil bds-c(18) (200 mm x 4. 6 mm x 5 mu m) column in combination of a gradient elution. the complete baseline resolution for 20 amino acid derivatives was achieved within 65 min. identification of amino acid derivatives was carried out by the online post-column ion trap/mass spectrometry with electrospray ion source (esi source) in positive ion mode. excellent linear response was observed, with coefficients of >0.9992. fluorescent detection limits calculated from 25 pmol injection, at a signal-to-noise ratio of 3, were 2.6 - 24 fmol. the established method for the determination of amino acids from cicada sample was satisfactory. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; hplc ; phthaldialdehyde ; system |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280677000001 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1674]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Xiao-Yan,You Jin-Mao,Sun Zhi-Wei,et al. Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification[J]. chinese journal of analytical chemistry,2010,38(7):917-923. |
| APA | Li Xiao-Yan.,You Jin-Mao.,Sun Zhi-Wei.,Fu Yan-Yan.,Qin Xue-Qin.,...&Li Yu-Lin.(2010).Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification.chinese journal of analytical chemistry,38(7),917-923. |
| MLA | Li Xiao-Yan,et al."Application of 2-[2-(7H-Dibenzo[a,g]-carbazol-7-yl)-ethoxyl] ethyl Chloroformate as A Pre-column Derivatization Reagent for Determination of Amino Acids from Hydrolyzed Cicada by High Performance Liquid Chromatography with Fluorescence Detection and Mass Spectrometric Identification".chinese journal of analytical chemistry 38.7(2010):917-923. |
入库方式： OAI收割
来源：西北高原生物研究所
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