HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau
文献类型：期刊论文
| 作者 | Yue, Pengpeng1,2; Sun, Jing1; Zhang, Changxian1,2; Ye, Runrong1; Lu, Xuefeng1; Zhou, Yubi1; Yang, Shibing1,2; Peng, Min1 |
| 刊名 | journal of medicinal plants research
 |
| 出版日期 | 2010-06-04 |
| 卷号 | 4期号:11页码:1053-1058 |
| 关键词 | HPLC-DAD flavonoids Meconopsis quintuplinervia Tibetan medicine |
| ISSN号 | 1996-0875 |
| 中文摘要 | this study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional tibetan medicine meconopsis quintuplinervia collecting from 29 different localities of qinghai-tibet plateau by high-performance liquid chromatography coupled with diode array detection (hplc-dad). the optimal conditions of separation and detection were achieved on a zorbax eclipse xdb-c(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. the complete separation was obtained within 40 min for the four analytes. the recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. the limits of detection (lod) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, s/n = 3:1) between 0.09 and 0.36 mu g ml(-1). furthermore, the main characteristics of flavonoids in m. quintuplinervia were also analyzed by principle component analysis (pca). |
| 英文摘要 | this study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional tibetan medicine meconopsis quintuplinervia collecting from 29 different localities of qinghai-tibet plateau by high-performance liquid chromatography coupled with diode array detection (hplc-dad). the optimal conditions of separation and detection were achieved on a zorbax eclipse xdb-c(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. the complete separation was obtained within 40 min for the four analytes. the recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. the limits of detection (lod) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, s/n = 3:1) between 0.09 and 0.36 mu g ml(-1). furthermore, the main characteristics of flavonoids in m. quintuplinervia were also analyzed by principle component analysis (pca). |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | chemistry, medicinal |
| 研究领域[WOS] | pharmacology & pharmacy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280356500009 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1680]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Res Ctr Natl Tibetan Med, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Pengpeng,Sun, Jing,Zhang, Changxian,et al. HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau[J]. journal of medicinal plants research,2010,4(11):1053-1058. |
| APA | Yue, Pengpeng.,Sun, Jing.,Zhang, Changxian.,Ye, Runrong.,Lu, Xuefeng.,...&Peng, Min.(2010).HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau.journal of medicinal plants research,4(11),1053-1058. |
| MLA | Yue, Pengpeng,et al."HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau".journal of medicinal plants research 4.11(2010):1053-1058. |
入库方式： OAI收割
来源：西北高原生物研究所
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