Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato (Solanum tuberosum L.)
文献类型：期刊论文
| 作者 | Zhang, B. O.1,2; Wang, Haiqing1; Shen, Yuhu1; Zhang, Huaigang1 |
| 刊名 | african journal of biotechnology
 |
| 出版日期 | 2010-05-03 |
| 卷号 | 9期号:18页码:2606-2612 |
| 关键词 | NOS Solanum tuberosum L. AtNOA1 |
| ISSN号 | 1684-5315 |
| 中文摘要 | in this study, the cdna of a noa (nitric oxide associated factor) was isolated with the strategy of est in silico cloning in combination with rt-pcr and 3'race from potato (solanum tuberosum l.) for the first time (named stnoa1). the stnoa1 bears a centrally positioned gtpase-binding domain. sequence alignment and phylogenetic analysis of the deduced stnoa1 protein with other known noa family protein indicates that stnoa1 is highly homologenous with nbnoa1 (noa from nicotiana benthamiana). the cdna was cloned into prokaryotic expression vector, pet-30a (+) and expressed in escherichia coli bl21 (de3) after induction with iptg. the recombinant protein was dissolved by 8 m urea and recovered by dialysis due to most of them were in inclusion bodies. then the recovered recombinant protein was purified by ni-nta and analyzed by sds-page. the results of sds-page showed that the stnoa1 was successfully expressed with the pet prokaryotic expression system and purified. the present study is the basis for further elucidating the biochemical characteristics of stnoa1 and is very significant for elucidating the nature of plant noa and its action mechanisms in endogenous no synthesis in plant species. |
| 英文摘要 | in this study, the cdna of a noa (nitric oxide associated factor) was isolated with the strategy of est in silico cloning in combination with rt-pcr and 3'race from potato (solanum tuberosum l.) for the first time (named stnoa1). the stnoa1 bears a centrally positioned gtpase-binding domain. sequence alignment and phylogenetic analysis of the deduced stnoa1 protein with other known noa family protein indicates that stnoa1 is highly homologenous with nbnoa1 (noa from nicotiana benthamiana). the cdna was cloned into prokaryotic expression vector, pet-30a (+) and expressed in escherichia coli bl21 (de3) after induction with iptg. the recombinant protein was dissolved by 8 m urea and recovered by dialysis due to most of them were in inclusion bodies. then the recovered recombinant protein was purified by ni-nta and analyzed by sds-page. the results of sds-page showed that the stnoa1 was successfully expressed with the pet prokaryotic expression system and purified. the present study is the basis for further elucidating the biochemical characteristics of stnoa1 and is very significant for elucidating the nature of plant noa and its action mechanisms in endogenous no synthesis in plant species. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | stomatal closure ; 2 ecotypes ; synthase ; arabidopsis ; plants ; responses ; signal ; involvement ; resistance ; protects |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277847400003 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1708]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, B. O.,Wang, Haiqing,Shen, Yuhu,et al. Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato (Solanum tuberosum L.)[J]. african journal of biotechnology,2010,9(18):2606-2612. |
| APA | Zhang, B. O.,Wang, Haiqing,Shen, Yuhu,&Zhang, Huaigang.(2010).Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato (Solanum tuberosum L.).african journal of biotechnology,9(18),2606-2612. |
| MLA | Zhang, B. O.,et al."Isolation, analysis and prokaryotic expression of nitric oxide associated factor gene-NOA1 from potato (Solanum tuberosum L.)".african journal of biotechnology 9.18(2010):2606-2612. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。