Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification
文献类型：期刊论文
| 作者 | Fu Yanyan1; Song Cuihua1; Qin Xueqin1; Li Xiaoyan1; Ding Mingzhou1; Sun Zhiwei2,3; Xia Lian1,2,3; Suo Yourui2; Li Yulin2; You Jinmao1,2 |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2010-04-14 |
| 卷号 | 68期号:7页码:653-660 |
| 关键词 | high performance liquid chromatography/ion-trap mass spectrometry percent ionization 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate (BAETS) bile acid |
| ISSN号 | 0567-7351 |
| 中文摘要 | on a reversed-phased hypersil c(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12h)-yl)ethyl 4-toluenesulfonate (baets) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with baets at 95 degrees c for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. the optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. the percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. the identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/ml for the labeled bile acids. |
| 英文摘要 | on a reversed-phased hypersil c(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12h)-yl)ethyl 4-toluenesulfonate (baets) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with baets at 95 degrees c for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. the optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. the percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. the identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/ml for the labeled bile acids. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; fatty-acids ; precolumn derivatization ; carboxylic-acids ; trifluoromethanesulfonate ; derivatives ; bryophyte ; reagent ; column ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277418700009 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1722]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu Yanyan,Song Cuihua,Qin Xueqin,et al. Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification[J]. acta chimica sinica,2010,68(7):653-660. |
| APA | Fu Yanyan.,Song Cuihua.,Qin Xueqin.,Li Xiaoyan.,Ding Mingzhou.,...&You Jinmao.(2010).Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification.acta chimica sinica,68(7),653-660. |
| MLA | Fu Yanyan,et al."Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification".acta chimica sinica 68.7(2010):653-660. |
入库方式： OAI收割
来源：西北高原生物研究所
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