Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification
文献类型：期刊论文
| 作者 | Xia, Lian1,2,3; Song, Cuihua1; Sun, Zhiwei2,3; Li, Guoliang2,3; Suo, Yourui2; You, Jinmao1,2 |
| 刊名 | chromatographia
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 71期号:7-8页码:623-631 |
| 关键词 | Column liquid chromatography-mass spectrometry Fluorescence detection Free fatty acids 1,2-Benzo-carbazole-9-ethyl-p-toluenesulfonate Potentilla anserina L. |
| ISSN号 | 0009-5893 |
| 中文摘要 | a sensitive method for the determination of free fatty acids using 1,2-benzo-carbazole-9-ethyl-p-toluenesulfonate (bcets) as tagging reagent with fluorescence detection has been developed. bcets could easily and quickly label fatty acids in the presence of the k(2)co(3) catalyst at 80 a degrees c for 30 min in n,n-dimethylformamide solvent. in this study, fatty acids from the extracted potentilla anserina l. plant sample were sensitively determined. the corresponding derivatives were separated on a reversed-phase eclipse xdb-c(8) column by lc in conjunction with gradient elution. the identification was carried out by post-column apci-ms in positive-ion detection mode. bcets-fatty acid derivatives gave an intense molecular ion peak at m/z [m+h](+), the collision-induced dissociation spectra of m/z [m+h](+) produced the specific fragment ions at m/z [m'+ch(2)ch(2)](+), m/z 216.6 and m/z [mh-h(2)o](+) (here, m': corresponding molecular mass of the fatty acids). the fluorescence excitation and emission wavelengths of the derivatives were at lambda (ex) 279 nm and lambda (em) 380 nm, respectively. linear correlation coefficients for all fatty acid derivatives are more than 0.9994. detection limits, at a signal-to-noise ratio of 3:1, are 10.79-34.19 fmol for the labeled fatty acids. |
| 英文摘要 | a sensitive method for the determination of free fatty acids using 1,2-benzo-carbazole-9-ethyl-p-toluenesulfonate (bcets) as tagging reagent with fluorescence detection has been developed. bcets could easily and quickly label fatty acids in the presence of the k(2)co(3) catalyst at 80 a degrees c for 30 min in n,n-dimethylformamide solvent. in this study, fatty acids from the extracted potentilla anserina l. plant sample were sensitively determined. the corresponding derivatives were separated on a reversed-phase eclipse xdb-c(8) column by lc in conjunction with gradient elution. the identification was carried out by post-column apci-ms in positive-ion detection mode. bcets-fatty acid derivatives gave an intense molecular ion peak at m/z [m+h](+), the collision-induced dissociation spectra of m/z [m+h](+) produced the specific fragment ions at m/z [m'+ch(2)ch(2)](+), m/z 216.6 and m/z [mh-h(2)o](+) (here, m': corresponding molecular mass of the fatty acids). the fluorescence excitation and emission wavelengths of the derivatives were at lambda (ex) 279 nm and lambda (em) 380 nm, respectively. linear correlation coefficients for all fatty acid derivatives are more than 0.9994. detection limits, at a signal-to-noise ratio of 3:1, are 10.79-34.19 fmol for the labeled fatty acids. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; carboxylic-acids ; derivatization reagent ; mass-spectrometry ; gas-chromatography ; trifluoromethanesulfonate ; chain ; oil ; 9-anthryldiazomethane ; derivatives |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277710400010 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1724]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shan Dong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Lian,Song, Cuihua,Sun, Zhiwei,et al. Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification[J]. chromatographia,2010,71(7-8):623-631. |
| APA | Xia, Lian,Song, Cuihua,Sun, Zhiwei,Li, Guoliang,Suo, Yourui,&You, Jinmao.(2010).Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification.chromatographia,71(7-8),623-631. |
| MLA | Xia, Lian,et al."Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification".chromatographia 71.7-8(2010):623-631. |
入库方式： OAI收割
来源：西北高原生物研究所
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