Extraction of Channel Catfish Muscle Oil by Supercritical Carbon Dioxide and Determination of Fatty Acids by Gas Chromatography-Electron Ionization-Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhang Feng-Ping1,2,3; Liu Yao-Min2; Shi Jun-You1,3; Wang Hong-Lun1; Zhao Xian-En1,3; Suo You-Rui1 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 38期号:2页码:163-168 |
| 关键词 | Supercritical carbon dioxide fluid extraction Gas chromatography-electron ionization mass spectrometry Fatty acid Channel catfish |
| ISSN号 | 0253-3820 |
| 中文摘要 | a novel method was established for the qualitative and quantitative determination of fatty acids in channel catfish muscle by gas chromatography-electron ionzation-mass spectrometry (gc-ei-ms) after super-critical carbon dioxide fluid extraction (sfe-co(2)). the extraction parameters for the methodology were optimized. the optimal conditions were extraction pressure of 25 mpa at 45 degrees c and extraction time of 100 min at the rate of carbon dioxide 30 l/h. the fatty acids in the muscle oil were derived by boron-trifluoride method. the saponification time was 10 min, and the esterication time was 20 min. the obtained fatty acid methylesters were separated by gas chromatography using a hp-innowax capillary column, and were detected by electron ionization mass spectrometry. full scan mode and sim mode were used for the qualitative and quantitative analysis, respectively. in the sim mode, saturated fatty acids were determined with m/z 74, mono-unsaturated fatty acids were determined with m/z 55, double-unsaturated fatty acids were determined with m/z 67, and polyunsaturated fatty acids were determined with m/z 79. the detection limits of 14 fatty acids were 2.2-20.0 mu g/l (s/n = 3), and the quantitative limits were 7.39-59.85 mu g/l (s/n = 10). the recoveries fell in the range from 90.0% to 111.2% (n = 4), and the relative standard deviation was between 2.0% and 5.9%. this effective, sensitive and reproducible method can be used for the determination of fatty acids in channel catfish muscle sample. |
| 英文摘要 | a novel method was established for the qualitative and quantitative determination of fatty acids in channel catfish muscle by gas chromatography-electron ionzation-mass spectrometry (gc-ei-ms) after super-critical carbon dioxide fluid extraction (sfe-co(2)). the extraction parameters for the methodology were optimized. the optimal conditions were extraction pressure of 25 mpa at 45 degrees c and extraction time of 100 min at the rate of carbon dioxide 30 l/h. the fatty acids in the muscle oil were derived by boron-trifluoride method. the saponification time was 10 min, and the esterication time was 20 min. the obtained fatty acid methylesters were separated by gas chromatography using a hp-innowax capillary column, and were detected by electron ionization mass spectrometry. full scan mode and sim mode were used for the qualitative and quantitative analysis, respectively. in the sim mode, saturated fatty acids were determined with m/z 74, mono-unsaturated fatty acids were determined with m/z 55, double-unsaturated fatty acids were determined with m/z 67, and polyunsaturated fatty acids were determined with m/z 79. the detection limits of 14 fatty acids were 2.2-20.0 mu g/l (s/n = 3), and the quantitative limits were 7.39-59.85 mu g/l (s/n = 10). the recoveries fell in the range from 90.0% to 111.2% (n = 4), and the relative standard deviation was between 2.0% and 5.9%. this effective, sensitive and reproducible method can be used for the determination of fatty acids in channel catfish muscle sample. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; bakery products ; methyl-esters ; quantification ; identification ; spectroscopy ; separation ; food |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275568300004 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1748]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Tongwei Co Ltd, Chengdu 610041, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Feng-Ping,Liu Yao-Min,Shi Jun-You,et al. Extraction of Channel Catfish Muscle Oil by Supercritical Carbon Dioxide and Determination of Fatty Acids by Gas Chromatography-Electron Ionization-Mass Spectrometry[J]. chinese journal of analytical chemistry,2010,38(2):163-168. |
| APA | Zhang Feng-Ping,Liu Yao-Min,Shi Jun-You,Wang Hong-Lun,Zhao Xian-En,&Suo You-Rui.(2010).Extraction of Channel Catfish Muscle Oil by Supercritical Carbon Dioxide and Determination of Fatty Acids by Gas Chromatography-Electron Ionization-Mass Spectrometry.chinese journal of analytical chemistry,38(2),163-168. |
| MLA | Zhang Feng-Ping,et al."Extraction of Channel Catfish Muscle Oil by Supercritical Carbon Dioxide and Determination of Fatty Acids by Gas Chromatography-Electron Ionization-Mass Spectrometry".chinese journal of analytical chemistry 38.2(2010):163-168. |
入库方式： OAI收割
来源：西北高原生物研究所
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