An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification
文献类型：期刊论文
| 作者 | You, Jinmao1,2; Song, Cuihua1; Yan, Tan1; Sun, Zhiwei2,3; Li, Yulin2; Suo, Yourui2 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2010-01-18 |
| 卷号 | 658期号:1页码:98-105 |
| 关键词 | Derivatization Fluorescence Amines 2-[2-(Dibenzocarbazole)-ethoxy] ethyl chloroformate Atmospheric chemical ionization-mass spectrometry |
| ISSN号 | 0003-2670 |
| 中文摘要 | an improved reagent named 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (dbcec-cl) for the determination of aliphatic amines by high-performance liquid chromatography (hplc) with fluorescence detection and post-column online atmospheric chemical ionization-mass spectrometry (apci-ms) identification has been developed. dbcec-cl could easily and quickly label aliphatic amines. derivatives were stable enough to be efficiently analyzed by hplc and showed an intense protonated molecular ion corresponding m/z [m+h](+) under apci-ms in positive-ion mode. the ratios for fluorescence responses were i(dbcec-amine)/i(bce-amine) = 1.02-1.60; i(dbcec-amine)/i(bceoc-amine) = 1.30-2.57: and i(dbcec-amine)/i(fmoc-amine) = 2.20-4.12 (here, i was relative fluorescence intensity). the ratios for ms responses were ic(dbcec-amine)/ic(bcec-amine) = 4.16-29.31 and ic(dbcec-amine)/ic(bceoc-amine) = 1.23-2.47 (here, ic: apci-ms ion current intensity). detection limits calculated from 0.0244 pmol injection, at a signal-to-noise ratio of 3, were 0.3-3.0 fmol. the relative standard deviations for within-day determination (n=6) were 0.045-0.081% for retention time and 0.86-1.03% for peak area for the tested aliphatic amines. the mean intra- and inter-assay precision for all amine levels were <3.64% and 4.67%, respectively. the mean recoveries ranged from 96.9% to 104.7% with their standard deviations in the range of 1.80-2.70 (rsds%). excellent linear responses were observed with coefficients of >0.9991. (c) 2009 elsevier b.v. all rights reserved. |
| 英文摘要 | an improved reagent named 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (dbcec-cl) for the determination of aliphatic amines by high-performance liquid chromatography (hplc) with fluorescence detection and post-column online atmospheric chemical ionization-mass spectrometry (apci-ms) identification has been developed. dbcec-cl could easily and quickly label aliphatic amines. derivatives were stable enough to be efficiently analyzed by hplc and showed an intense protonated molecular ion corresponding m/z [m+h](+) under apci-ms in positive-ion mode. the ratios for fluorescence responses were i(dbcec-amine)/i(bce-amine) = 1.02-1.60; i(dbcec-amine)/i(bceoc-amine) = 1.30-2.57: and i(dbcec-amine)/i(fmoc-amine) = 2.20-4.12 (here, i was relative fluorescence intensity). the ratios for ms responses were ic(dbcec-amine)/ic(bcec-amine) = 4.16-29.31 and ic(dbcec-amine)/ic(bceoc-amine) = 1.23-2.47 (here, ic: apci-ms ion current intensity). detection limits calculated from 0.0244 pmol injection, at a signal-to-noise ratio of 3, were 0.3-3.0 fmol. the relative standard deviations for within-day determination (n=6) were 0.045-0.081% for retention time and 0.86-1.03% for peak area for the tested aliphatic amines. the mean intra- and inter-assay precision for all amine levels were <3.64% and 4.67%, respectively. the mean recoveries ranged from 96.9% to 104.7% with their standard deviations in the range of 1.80-2.70 (rsds%). excellent linear responses were observed with coefficients of >0.9991. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; volatile n-nitrosamines ; derivatizing reagent ; biogenic-amines ; 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate ; precolumn derivatization ; labeling reagent ; enzymatic method ; water samples ; chloroformate |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273847500014 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1754]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Song, Cuihua,Yan, Tan,et al. An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification[J]. analytica chimica acta,2010,658(1):98-105. |
| APA | You, Jinmao,Song, Cuihua,Yan, Tan,Sun, Zhiwei,Li, Yulin,&Suo, Yourui.(2010).An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification.analytica chimica acta,658(1),98-105. |
| MLA | You, Jinmao,et al."An improved reagent for determination of aliphatic amines with fluorescence and online atmospheric chemical ionization-mass spectrometry identification".analytica chimica acta 658.1(2010):98-105. |
入库方式： OAI收割
来源：西北高原生物研究所
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