DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT
文献类型：期刊论文
| 作者 | Sun, Zhiwei1,3; Xia, Lian1,2,3; Suo, Yourui1; You, Jinmao1,2 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:3页码:390-404 |
| 关键词 | 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (DBCPC-Cl) aliphatic amines derivatization fluorescent probe HPLC |
| ISSN号 | 1082-6076 |
| 中文摘要 | a novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (dbcpc-cl) has been designed and synthesized for amine labeling in hplc. using 12 aliphatic amines as the models, the derivatization conditions were optimized. in 0.2mol/l borate buffer (ph 9.0), amines reacted with dbcpc-cl at 30 degrees c to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by hplc. the separation of these amine derivatives was achieved with a c8 column and gradient elution by using 30% acetonitrile (containing 20mmol/l formic acid) and 100% acetonitrile, and online apci/ms identification of the derivatives was carried out in positive-ion mode. the fluorescence responses for dbcpc-derivatives were higher relative to those obtained using previously reported reagents. with fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). the relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. the mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. the mean recoveries ranged from 86.9 to 104.7%. excellent linear responses were observed with coefficients >0.9991. |
| 英文摘要 | a novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (dbcpc-cl) has been designed and synthesized for amine labeling in hplc. using 12 aliphatic amines as the models, the derivatization conditions were optimized. in 0.2mol/l borate buffer (ph 9.0), amines reacted with dbcpc-cl at 30 degrees c to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by hplc. the separation of these amine derivatives was achieved with a c8 column and gradient elution by using 30% acetonitrile (containing 20mmol/l formic acid) and 100% acetonitrile, and online apci/ms identification of the derivatives was carried out in positive-ion mode. the fluorescence responses for dbcpc-derivatives were higher relative to those obtained using previously reported reagents. with fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). the relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. the mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. the mean recoveries ranged from 86.9 to 104.7%. excellent linear responses were observed with coefficients >0.9991. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; precolumn derivatization ; biogenic-amines ; fluorescence detection ; acids ; derivatives ; samples |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274174400008 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1778]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal, Coll Chem Sci, Qufu, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,Xia, Lian,Suo, Yourui,et al. DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT[J]. journal of liquid chromatography & related technologies,2010,33(3):390-404. |
| APA | Sun, Zhiwei,Xia, Lian,Suo, Yourui,&You, Jinmao.(2010).DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT.journal of liquid chromatography & related technologies,33(3),390-404. |
| MLA | Sun, Zhiwei,et al."DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT".journal of liquid chromatography & related technologies 33.3(2010):390-404. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。