4-(1-METHYL-1H-PHENANTHRO[9,10-d]IMIDAZOLE-2-) BENZOIC ACID (MPIBA) AND ITS APPLICATION FOR DETERMINATION OF AMINES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROSCOPY/ATMOSPHERIC PRESSURE CHEMICAL IONIZATION
文献类型：期刊论文
| 作者 | Xia Lian1,2,3; Sun Zhiwei2,3; Suo Yourui2; You Jinmao1,2 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:1页码:72-88 |
| 关键词 | 4-(1-methyl-1H-phenanthro[9 10-d]imidazole-2-) benzoic acid (MPIBA) amines determination fluorescence detection HPLC mass spectrometry |
| ISSN号 | 1082-6076 |
| 中文摘要 | a simple, sensitive, and mild method for the determination of amines based on a condensation reaction with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc center dot hcl) as the dehydrant with fluorescence detection has been developed. amines were derivatized to their acid amides with labeling reagent 4-s(1-methyl-1h-phenanthro[9,10-d]imidazole-2-) benzoic acid (mpiba). studies on derivatization conditions indicated that the coupling reaction proceeded rapidly and smoothly in acetonitrile to give the corresponding sensitively fluorescent derivatives with a maximum excitation at ex 260nm and a maximum emission at em 446nm. the labeled derivatives exhibited high stability and were enough to be efficiently analyzed by high-performance liquid chromatography. identification of derivatives was carried out by online post-column mass spectrometry (lc/apci-ms/ms) and showed an intense protonated molecular ion corresponding m/z [mh]+ under apci in positive-ion mode. at the same time, the fluorescence properties of derivatives in various solvents were investigated. the method, in conjunction with a gradient elution, offered a baseline resolution of the common amine derivatives on a reversed-phase eclipse xdb-c8 column. liquid chromatography separation for the derivatized amines showed good reproducibility with acetonitrile-water as mobile phase. detection limits calculated from 90.00pmol to 88fmol injection, at a signal-to-noise ratio of 3, were 10.5-53.4fmol. excellent linear responses were observed with coefficients of 0.9996. the established method for the determination of aliphatic amines from real wastewater was satisfactory. |
| 英文摘要 | a simple, sensitive, and mild method for the determination of amines based on a condensation reaction with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (edc center dot hcl) as the dehydrant with fluorescence detection has been developed. amines were derivatized to their acid amides with labeling reagent 4-s(1-methyl-1h-phenanthro[9,10-d]imidazole-2-) benzoic acid (mpiba). studies on derivatization conditions indicated that the coupling reaction proceeded rapidly and smoothly in acetonitrile to give the corresponding sensitively fluorescent derivatives with a maximum excitation at ex 260nm and a maximum emission at em 446nm. the labeled derivatives exhibited high stability and were enough to be efficiently analyzed by high-performance liquid chromatography. identification of derivatives was carried out by online post-column mass spectrometry (lc/apci-ms/ms) and showed an intense protonated molecular ion corresponding m/z [mh]+ under apci in positive-ion mode. at the same time, the fluorescence properties of derivatives in various solvents were investigated. the method, in conjunction with a gradient elution, offered a baseline resolution of the common amine derivatives on a reversed-phase eclipse xdb-c8 column. liquid chromatography separation for the derivatized amines showed good reproducibility with acetonitrile-water as mobile phase. detection limits calculated from 90.00pmol to 88fmol injection, at a signal-to-noise ratio of 3, were 10.5-53.4fmol. excellent linear responses were observed with coefficients of 0.9996. the established method for the determination of aliphatic amines from real wastewater was satisfactory. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | aliphatic-amines ; biogenic-amines ; derivatization ; spectrometry ; methanol ; serum |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272680200006 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1780]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shan Dong Prov, Qufu 273165, Shandong, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia Lian,Sun Zhiwei,Suo Yourui,et al. 4-(1-METHYL-1H-PHENANTHRO[9,10-d]IMIDAZOLE-2-) BENZOIC ACID (MPIBA) AND ITS APPLICATION FOR DETERMINATION OF AMINES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROSCOPY/ATMOSPHERIC PRESSURE CHEMICAL IONIZATION[J]. journal of liquid chromatography & related technologies,2010,33(1):72-88. |
| APA | Xia Lian,Sun Zhiwei,Suo Yourui,&You Jinmao.(2010).4-(1-METHYL-1H-PHENANTHRO[9,10-d]IMIDAZOLE-2-) BENZOIC ACID (MPIBA) AND ITS APPLICATION FOR DETERMINATION OF AMINES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROSCOPY/ATMOSPHERIC PRESSURE CHEMICAL IONIZATION.journal of liquid chromatography & related technologies,33(1),72-88. |
| MLA | Xia Lian,et al."4-(1-METHYL-1H-PHENANTHRO[9,10-d]IMIDAZOLE-2-) BENZOIC ACID (MPIBA) AND ITS APPLICATION FOR DETERMINATION OF AMINES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND IDENTIFICATION WITH MASS SPECTROSCOPY/ATMOSPHERIC PRESSURE CHEMICAL IONIZATION".journal of liquid chromatography & related technologies 33.1(2010):72-88. |
入库方式： OAI收割
来源：西北高原生物研究所
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