LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
文献类型：期刊论文
| 作者 | Sun, Jing1; Li, Faqiang2; Xu, Wenhua1; Zhou, Guoying1; You, Jinmao1,3; Chen, Guichen1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 70期号:11-12页码:1627-1633 |
| 关键词 | Column liquid chromatography LC-ESI-MS Free amino acids Tibetan medicine Gentiana dahurica |
| ISSN号 | 0009-5893 |
| 中文摘要 | concentrations of 20 free amino aicds (faas) in a famous tibetan medicine gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (bceoc) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (rp-lc). 20 amino acid derivatives (aad) were separated on a hypersil bds c(18) column with a good baseline resolution within 65 min. identification of 20 aad was by online post-column mass spectrometry with an electrospray ionization (esi) source. the validation of the method was examined by linearity, repeatability, and detection limits. most linear correlation coefficients for aad were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. there were 18 faas found in g. dahurica, of which seven faas were necessary to the people's health and related to the treatment of liver and gall disease. variation of concentrations of the 20 faas showed geographical distribution difference among populations. meanwhile a stable genetic diversity of faas composition of g. dahurica was also revealed at the species level. results of the present study proved that the established method was rapid and reproducible for further separation and determination of faas in more medicinal plants. |
| 英文摘要 | concentrations of 20 free amino aicds (faas) in a famous tibetan medicine gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (bceoc) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (rp-lc). 20 amino acid derivatives (aad) were separated on a hypersil bds c(18) column with a good baseline resolution within 65 min. identification of 20 aad was by online post-column mass spectrometry with an electrospray ionization (esi) source. the validation of the method was examined by linearity, repeatability, and detection limits. most linear correlation coefficients for aad were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. there were 18 faas found in g. dahurica, of which seven faas were necessary to the people's health and related to the treatment of liver and gall disease. variation of concentrations of the 20 faas showed geographical distribution difference among populations. meanwhile a stable genetic diversity of faas composition of g. dahurica was also revealed at the species level. results of the present study proved that the established method was rapid and reproducible for further separation and determination of faas in more medicinal plants. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; mass-spectrometry ; chloroformate |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272173800016 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1786]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Natl Tibetan Med, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jing,Li, Faqiang,Xu, Wenhua,et al. LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization[J]. chromatographia,2009,70(11-12):1627-1633. |
| APA | Sun, Jing,Li, Faqiang,Xu, Wenhua,Zhou, Guoying,You, Jinmao,&Chen, Guichen.(2009).LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization.chromatographia,70(11-12),1627-1633. |
| MLA | Sun, Jing,et al."LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization".chromatographia 70.11-12(2009):1627-1633. |
入库方式： OAI收割
来源：西北高原生物研究所
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