High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans
文献类型：期刊论文
| 作者 | Dou, Quan-Wen1; Chen, Zhi-Guo1; Liu, Yong-An1; Tsujimoto, Hisashi2 |
| 刊名 | breeding science
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 59期号:5页码:651-656 |
| 关键词 | perennial grass forage Elymus nutans karyotype variation FISH GISH |
| ISSN号 | 1344-7610 |
| 中文摘要 | elymus nutans l. (sthy, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the qinghai-tibet plateau in china due to its high tolerance to environmental stresses, such as cold and drought. karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (fish) and genomic in situ hybridization (gish). gish discriminated st, h and y genomes, indicating that e. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. however, fish using an agg satellite and afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. in addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. the nature of the polymorphisms seen in the chromosomes of the domesticated population of e. nutans is discussed. |
| 英文摘要 | elymus nutans l. (sthy, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the qinghai-tibet plateau in china due to its high tolerance to environmental stresses, such as cold and drought. karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (fish) and genomic in situ hybridization (gish). gish discriminated st, h and y genomes, indicating that e. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. however, fish using an agg satellite and afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. in addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. the nature of the polymorphisms seen in the chromosomes of the domesticated population of e. nutans is discussed. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | agronomy ; plant sciences |
| 研究领域[WOS] | agriculture ; plant sciences |
| 关键词[WOS] | in-situ hybridization ; repetitive sequences ; afa-family ; triticeae ; poaceae ; identification ; genome ; barley ; leymus ; wheat |
| 收录类别 | SCI ; ISTP |
| 语种 | 英语 |
| WOS记录号 | WOS:000274757200026 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1788]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Tottori Univ, Fac Agr, Tottori 6808550, Japan |
| 推荐引用方式 GB/T 7714 | Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,et al. High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J]. breeding science,2009,59(5):651-656. |
| APA | Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,&Tsujimoto, Hisashi.(2009).High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans.breeding science,59(5),651-656. |
| MLA | Dou, Quan-Wen,et al."High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans".breeding science 59.5(2009):651-656. |
入库方式： OAI收割
来源：西北高原生物研究所
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