Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy
文献类型：期刊论文
| 作者 | Sun, Zhiwei1,3; You, Jinmao1,2; Xia, Lian1,3; Suo, Yourui1 |
| 刊名 | chromatographia
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 70期号:7-8页码:1055-1063 |
| 关键词 | Column liquid chromatographia Mass spectrometry Pre-column derivatization Fluorescence detection Aliphatic amines |
| ISSN号 | 0009-5893 |
| 中文摘要 | 4-(1h-phenanthro[9,10-d]imidazol-2-yl)benzoic acid (piba), a new amine-reactive fluorescent probe was synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines from environmetal water samples. derivatization was successfully carried out by a condensation reaction using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride as the dehydrant. studies on derivatization conditions indicated that the condensation reaction proceeded rapidly and smoothly in the presence of pyridine. amines were labeled with piba to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda (ex) 260 nm and an emission maximum at lambda (em) 445 nm. identification of derivatives was carried out by online apci-ms-ms and showed an intense protonated molecular ion corresponding m/z [m + h](+) in the positive ion mode. excellent linear responses were observed with coefficients of > 0.9996. detection limits were 6.7-27 fmol (at a signal-to-noise ratio of 3). the mean intra- and inter-assay precision for all amine levels were < 2.79 and 3.85%, respectively. the established method for the determination of aliphatic amines from real water was satisfactory. |
| 英文摘要 | 4-(1h-phenanthro[9,10-d]imidazol-2-yl)benzoic acid (piba), a new amine-reactive fluorescent probe was synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines from environmetal water samples. derivatization was successfully carried out by a condensation reaction using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride as the dehydrant. studies on derivatization conditions indicated that the condensation reaction proceeded rapidly and smoothly in the presence of pyridine. amines were labeled with piba to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda (ex) 260 nm and an emission maximum at lambda (em) 445 nm. identification of derivatives was carried out by online apci-ms-ms and showed an intense protonated molecular ion corresponding m/z [m + h](+) in the positive ion mode. excellent linear responses were observed with coefficients of > 0.9996. detection limits were 6.7-27 fmol (at a signal-to-noise ratio of 3). the mean intra- and inter-assay precision for all amine levels were < 2.79 and 3.85%, respectively. the established method for the determination of aliphatic amines from real water was satisfactory. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; solid-phase microextraction ; n-hydroxysuccinimide ester ; derivatizing reagent ; biogenic-amines ; waste-water ; 9-fluorenylmethyl chloroformate ; spectrometry ; separation ; chloride |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271069400006 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1820]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhiwei,You, Jinmao,Xia, Lian,et al. Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy[J]. chromatographia,2009,70(7-8):1055-1063. |
| APA | Sun, Zhiwei,You, Jinmao,Xia, Lian,&Suo, Yourui.(2009).Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy.chromatographia,70(7-8),1055-1063. |
| MLA | Sun, Zhiwei,et al."Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy".chromatographia 70.7-8(2009):1055-1063. |
入库方式： OAI收割
来源：西北高原生物研究所
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