Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp.
文献类型：会议论文
| 作者 | Hong-manHou ; Li-liZhai ; PengXu ; Yu XJ(虞星炬) ; Zhang W(张卫) |
| 出版日期 | 2008-10-12 |
| 会议名称 | 13th international biotechnology symposium and exhibition |
| 会议日期 | 2008-10-12 |
| 会议地点 | 中国 |
| 页码 | s597/2 |
| 通讯作者 | 张卫 |
| 中文摘要 | lipopeptide is a kind of biosurfactant synthesized by microorganisms. in addition to the classical application as surfactant,some unique bioactive functions were also being focused. marine sponges are well-known as conglomerates of various organisms. good oil emulsification effect was found during the enrichment culture of the sponge phakeliia sp. extracts. in this study, bacteria producing biosurfactant were isolated from the sponge by oil spreading technique, and the lipopeptide biosurfactant was extracted and characterized on the surface activity, physicochemical property and bioactivities (noha et al., 2004). 62 strains isolated from the sponge extracts were proved that three of them had the abilities to produce biosurfactant. the three isolated strain were designated as xp1, xp2, h10. 16s ribosomal dna sequencing revealed that strains xp1, xp2, h10 belonged to the genus pseudomonas, bacillus pumilus and bacillus subtilis, respectively. strain xp1 was selected for its best surface activity. its biosurfactant was identified as a type of lipopeptide and purified by sephadex lh-20 and ods column, named as xp-bs. the xp-bs lowered the surface tension of water to 26.5 dyn/cm, and the cmc was 40 m. the xp-bs was stable at ph 2.0–8.5, which was different from other biosurfactants (christian et al., 2003; stanislav et al., 2003). moreover, it showed great thermal stability at 100 ◦c and 121 after incubation for 2 h and 20 min, respectively. the function of xp-bs was not affected at a salt concentration of 1000mm for nacl and 10mm for cacl2. xp-bs was tested against a set of standard gram-positive, gram-negative, and eukaryotic microorganisms. it exhibited a considerable antimicrobial activity against staphylococcus aureus and escherichia coli, a lower activity against epiphyte. xp-bs also showed certain superiority to other chemically synthesized surfactants. all the above results suggested that the applications of xp-bs could be developed commercially. |
| 会议主办者 | 中国科学院,中国工程院,中国教育部,中国科技部,中国国家发改委 |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| 源URL | [http://159.226.238.44/handle/321008/113052]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Hong-manHou,Li-liZhai,PengXu,et al. Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp.[C]. 见:13th international biotechnology symposium and exhibition. 中国. 2008-10-12. |
入库方式： OAI收割
来源：大连化学物理研究所
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