Tandem immunoaffinity columns coupled with LC-MS/MS for the determination of isoproturon and its metabolite in river water
文献类型：会议论文
| 作者 | Zhang XL(张秀莉) ; DieterMartens ; PetraM.Krä ; mer ; AntoniusA.Ketrrup ; Liang XM(梁鑫淼) |
| 出版日期 | 2008-10-12 |
| 会议名称 | 13th international biotechnology symposium & exhibition |
| 会议日期 | 2008-10-12 |
| 会议地点 | 中国 |
| 页码 | s436/2 |
| 其他题名 | 串联免疫亲和柱与lc-ms/ms联用测定河水中异丙隆及其降解产物 |
| 通讯作者 | 梁鑫淼 |
| 中文摘要 | isoproturon is a widely used herbicide[1]. its first metabolite is mono-demethyl-isoproturon (d1m-ipo) in environmental condition. both of them may accumulate and achieve intolerable concentrations in drinking water [2]. the maximum admissible concentration in drinking water set by the european union is 0.1 μg/l. thus, a sensitive and selective method could be preferred for trace analysis of isoproturon and d1m-ipo in water. two kind of monoclonal anti-isoproturon antibodies were entrapped in a sol-gel matrix, respectively. depending on their affinity to isoproturon, the two immunoaffinity columns were named liac and hiac. the liac can concentrate the isoproturon in 25ml water with 90% recovery using 40% (v/v) acetonitrile (acn) as our previous described [3], but only with 9% recovery for d1m-ipo. the hiac can concentrate d1m-ipo in 25ml water with 95.6% recovery using 40% acn and isoproturon with 70% acn. however, the capacity of hiac after one time elution of 70% acn decreased from 120ng to 50ng, that mean the hiac can’t be reused for the concentration of isoproturon. after ensuring that isoproturon in 40% acn will not bind on the hiac, the liac was connected with hiac by a spe column adaptor to establish the tandem immunoaffinity extraction method. the liac-hiac was combined with liquid chromatography–tandem mass spectrometry to determine isoproturon and d1m-ipo simultaneously in surface water, and the linear range was up to 2.2 μg/l with correlation coefficient higher than 0.99 and more than 88% recoveries (n = 8). all of these data proved that tandem immunoaffinity columns were effective and reusable tool for extraction of isoproturon and d1m-ipo from aqueous matrix with good reliability and high selectivity. [1] tomlin, c.; (editor) 1994. the pesticide manual, 10th ed. ed.; royal society of chemistry and the british crop protection council: farnham, surrey, uk, [2] krämer, p. m.; kremmer, e.; forster, s.; goodrow, m. h. 2004, extending the working range of immunoanalysis by exploitation of two monoclonal antibodies j. agric. food chem. 52, 6394-6401. [3] zhang, x. l.; martens, d.; krämer, p. m.; kettrup, a. a.; liang, x. m. 2006, development and application of a sol–gel immunosorbent-based method for the determination of isoproturon in surface water j. chromatogr. a 1102, 84-90. |
| 会议主办者 | 中国科学院、中国工程院、教育部、科技部和国家发改委 |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| WOS记录号 | WOS:000208979401517 |
| 源URL | [http://159.226.238.44/handle/321008/113160]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang XL,DieterMartens,PetraM.Krä,et al. Tandem immunoaffinity columns coupled with LC-MS/MS for the determination of isoproturon and its metabolite in river water[C]. 见:13th international biotechnology symposium & exhibition. 中国. 2008-10-12. |
入库方式： OAI收割
来源：大连化学物理研究所
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