IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES
文献类型：会议论文
| 作者 | Yang F(杨帆) ; Zhang SF(张素芳) ; Tang W(唐伟) ; Zhao ZB(赵宗保) |
| 出版日期 | 2008-10-12 |
| 会议名称 | 13th International Biotechnology Symposium and Exhibition |
| 会议日期 | 2008-10-12 |
| 会议地点 | 中国 |
| 页码 | S323/2 |
| 通讯作者 | 赵宗保 |
| 中文摘要 | Rhodosporidium toruloides is an excellent microbial lipid producer of great industrial potential [1]; yet, there is no effective genetic tool for rational engineering this microorganism. Orotidine-5’-monophosphate decarboxylase (OMPdecase), usually encoded by the gene ura3, and has been widely employed as a genetic selection maker in Saccharomyces cerevisiae [2], other yeasts [3] and fungi. To develop a marker recycling system for R. toruloides, the ura3 gene of R. toruloides (Rt-ura3) was isolated using methods of degenerate PCR together with RACE. Results showed that the cDNA and genomic sequence for Rt-ura3 were 840 bp and 1017 bp, respectively, indication that the gene contained 4 extrons and 3 introns. The encoded polypeptide held an amino acid sequence of 279 residues with significant homology to those of OMPdecases from other yeasts. A shuttle vector pYES2/CT-Rt-ura3 was constructed via site-specific insertion of Rt-ura3 into the commercial vector pYES2/CT. Transformation of the vector into S. cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated Rt-ura3 was functionally equivalent to the ura3 gene from S. cerevisiae. Further work is going on in this laboratory to engineer oleaginous yeasts based on this information. |
| 会议主办者 | 中国科学院,中国工程院,中国教育部,中国科技部 |
| 学科主题 | 物理化学 |
| 语种 | 中文 |
| ISSN号 | 0168-1656 |
| 源URL | [http://159.226.238.44/handle/321008/113176]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Yang F,Zhang SF,Tang W,et al. IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12. |
入库方式： OAI收割
来源：大连化学物理研究所
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