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Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission

文献类型：期刊论文

作者Zhu, Shuyun4; Kong, Rongmei4; Liu, Wei1; Zuo, Ya-Nan4; Wang, Xiao1; Wang, Renjun2; Zhao, Xian-En4; Sun, Jing3; Yan, Xiaolu4
刊名MICROCHIMICA ACTA
出版日期2018-09-01
卷号185期号:9
ISSN号0026-3672
关键词Fluorescence Dna-templated Synthesis Silver Nanoclusters Bleomycin Guanine-rich Sequence Antitumor Drug Dna Scission
DOI10.1007/s00604-018-2939-7
文献子类Article
英文摘要The authors describe a molecular beacon-based fluorescent probe for the determination of the cancer drug bleomycin (BLM). The probe was tagged with DNA-templated silver nanoclusters (DNA-AgNCs) and guanine-rich sequences (GRSs) at two terminals serving as signal reporter with a loop. In the absence of the BLM-iron(II) complex [BLM-Fe(II)], the probe has a hairpin shape and displays strong fluorescence because the AgNCs are close to the GRSs. In the presence of the BLM-Fe(II) complex, it will selectively cleave the probe at the 5'-GC-3' scission site of the loop. This displaces the AgNCs away from the GRSs and causes a decrease in fluorescence, best measured at excitation/emission wavelengths of 565/623 nm. This effect enables BLM to be detected with a detection limit as low as 33 pM, which was 1-3 orders of magnitude more sensitive than most of the previous reports. The probe was applied for the determination of BLM in spiked human serum samples, and excellent performance was achieved. In our perception, the method described here represents a promising tool for highly sensitive and specific analysis of BLM during cancer treatment.
WOS关键词GLUTATHIONE-REDUCTASE ACTIVITY ; GRAPHENE QUANTUM DOTS ; TURN-ON DETECTION ; LABEL-FREE ; COPPER NANOCLUSTERS ; GOLD NANOPARTICLES ; SENSING PLATFORM ; QUANTIFICATION ; IDENTIFICATION ; AMPLIFICATION
WOS研究方向Chemistry
语种英语
出版者SPRINGER WIEN
WOS记录号WOS:000441049900002
资助机构National Natural Science Foundation of China(21405094 ; Natural Science Foundation of Shandong Province(ZR2017MC037 ; Natural Science Foundation of Qinghai Province of China(2016-ZJ-955) ; Development Project of Qinghai Key Laboratory(2017-ZJ-Y10) ; Open Funds of Shandong Key Laboratory of TCM Quality Control Technology of Shandong Academy of Sciences ; 21775088 ; ZR2017QB008) ; 81403051)
源URL[http://ir.nwipb.ac.cn/handle/363003/13766]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者Zhu, Shuyun
作者单位1.Qilu Univ Technol, Shandong Acad Sci, Shandong Anal & Test Ctr, Shandong Key Lab TCM Qual Control Technol, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China
2.Qufu Normal Univ, Coll Life Sci, Qufu 273165, Shandong, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China
4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Shuyun,Kong, Rongmei,Liu, Wei,et al. Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission[J]. MICROCHIMICA ACTA,2018,185(9).
APA Zhu, Shuyun.,Kong, Rongmei.,Liu, Wei.,Zuo, Ya-Nan.,Wang, Xiao.,...&Yan, Xiaolu.(2018).Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission.MICROCHIMICA ACTA,185(9).
MLA Zhu, Shuyun,et al."Molecular beacon-templated silver nanoclusters as a fluorescent probe for determination of bleomycin via DNA scission".MICROCHIMICA ACTA 185.9(2018).

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来源：西北高原生物研究所

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