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Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity

文献类型：期刊论文

作者Suo, Yourui2,3,5; Hu, Na2,5; Zhou, Haonan1,2,5; Deng, Kai1,2,5; Meng, Jing1,2,5; Zhou, Wu1,2,3,5; Chen, Shasha1,2,5; Zhang, Gong1,2,4,5
刊名FOOD CHEMISTRY
出版日期2018-12-15
卷号269页码:150-156
ISSN号0308-8146
关键词Lycium Ruthenicum Murray Orbitrap Ms Phenolic Compounds Antioxidant
DOI10.1016/j.foodchem.2018.06.132
文献子类Article
英文摘要Lycium ruthenicum Murray (LR) is a functional food, and it has long been used in traditional folk medicine. However, detailed qualitative and quantitative analyses related to its phenolic compounds remains scarce. This work reports, for the first time, the establishment of a rapid method for simultaneous identification and quantification of 25 phenolic compounds by UPLC-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). This method was validated by LODs, LOQs, precision, repeatability, stability, mean recovery, recovery range and RSD. The confirmed method was applied to the analysis of phenolic compounds in LR. Finally, 18 phenolic compounds in LR were qualitatively and quantitatively analyzed. Among them, 11 constituents were detected for the first time, which included two flavonoids (catechin and naringenin) and seven phenolic acids (gallic acid, vanillic acid, 2,4-dihydroxybenzoic acid, veratronic acid, benzoic acid, ellagic acid and salicylic acid). Moreover, Phloretin and protocatechuate, belonging to the dihydrochalcone flavonoid and protocatechuic acid respectively, were also identified and quantified. The total phenolics content (20.17 +/- 2.82 mg/g) and the total anthocyanin content (147.43 +/- 1.81 mg/g) were determined. In addition, the antioxidant activities of the LR extract were evaluated through 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and total antioxidant activity (T-AOC) assays.
WOS关键词SIGNALING PATHWAY ; OXIDATIVE STRESS ; CHLOROGENIC ACID ; INFLAMMATION ; FLAVONOIDS ; RUTIN ; NARINGENIN ; PHLORETIN ; QUERCETIN ; GROWTH
WOS研究方向Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000441142100019
资助机构Qinghai Provincial Science Foundation(2016-ZJ-928Q ; CAS, China "Light of West China" Program (2016): Study on the effect of Lycium ruthenicum Murr. anthocyanin on the liver protection, Capacity Building Project of Engineering Research Center of Qinghai Province(2017-GX-G03) ; Innovation Platform for the Development and Construction of Special Project of Key Laboratory of Tibetan Medicine Research of Qinghai Province(2017-ZJ-Y11) ; 2015-NK-509 ; 2015-SF-120)
源URL[http://ir.nwipb.ac.cn/handle/363003/13780]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者Suo, Yourui; Hu, Na
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Qinghai Prov Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
3.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China
4.Yanan Univ, Affiliated Hosp, Yanan 716000, Shaanxi, Peoples R China
5.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
推荐引用方式
GB/T 7714		 Suo, Yourui,Hu, Na,Zhou, Haonan,et al. Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity[J]. FOOD CHEMISTRY,2018,269:150-156.
APA Suo, Yourui.,Hu, Na.,Zhou, Haonan.,Deng, Kai.,Meng, Jing.,...&Zhang, Gong.(2018).Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity.FOOD CHEMISTRY,269,150-156.
MLA Suo, Yourui,et al."Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity".FOOD CHEMISTRY 269(2018):150-156.

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来源：西北高原生物研究所

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