miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis
文献类型：期刊论文
| 作者 | Fang, Xingtang2; Chen, Hong2; Hu, Linyong1 ; Zhang, Chunlei2; Wang, Yanhong2; Yang, Lingzhi2; Qi, Qi2; Tan, Jiaoyan2; Wang, Jian2
|
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
 |
| 出版日期 | 2018 |
| 卷号 | 14期号:7页码:760-774 |
| 关键词 | Mir-487b-3p Irs1 Myoblast Proliferation Myoblast Differentiation Myogenesis |
| ISSN号 | 1449-2288 |
| DOI | 10.7150/ijbs.25052 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs are endogenous, small non-coding RNAs that can play critical gene-regulatory roles during skeletal muscle development and are highly conserved. miR-487b-3p is expressed in muscle, and the detailed mechanism by which it regulates myoblast proliferation and differentiation has not been explored. Here, we found that miR-487b-3p expression was significantly higher in goat muscle tissues than in other tissues and was higher in fetal goat muscle tissues than in mature goat tissues, suggesting that miR-487b-3p has an important effect on skeletal muscle myogenesis. Functional studies showed that miR-487b-3p overexpression significantly suppressed C2C12 myoblast proliferation and differentiation, which was accompanied by the down-regulation of functional genes related to proliferation (MyoD, Pax7 and PCNA) and differentiation (Myf5, MyoG and Mef2c), whereas the inhibition of miR-487b-3p accelerated C2C12 myoblast proliferation and differentiation and was accompanied by the up-regulation of functional genes. Using Target-Scan and David, we found that miR-487b-3p targeted the 3'-UTR of IRS1, an essential regulator in the PI3K/Akt and MAPK/Erk pathways. We then confirmed the targeting of IRS1 by miR-487b-3p using dual-luciferase assays, RT-qPCR and western blotting. Furthermore, IRS1 silencing markedly inhibited proliferation and differentiation in cultured C2C12 myoblasts, confirming the important role of IRS1 in myogenesis. These results reveal an IRS1 -mediated regulatory link between miR-487b-3p and the PI3K/Akt and MAPK/Erk pathways during skeletal muscle myogenesis. |
| WOS关键词 | SATELLITE CELLS ; TRANSCRIPTION FACTORS ; PI3K/AKT PATHWAYS ; GROWTH-FACTOR ; EXPRESSION ; MICRORNAS ; MAPK/ERK ; CANCER ; PHOSPHORYLATION ; INHIBITION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000433261800007 |
| 出版者 | IVYSPRING INT PUBL |
| 资助机构 | Science and Technology Planning Project of Jiangsu Province(SZ-XZ2017016 ; Science and Technology Planning Project of Xuzhou City(KC16NX073 ; Natural Science Foundation of Jiangsu Province(BK20160217) ; Applied Basic Research Program of Qinghai Province(2014-ZJ-710) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; BN2015027) ; KC15N0013) |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13822]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 通讯作者 | Fang, Xingtang |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Xingtang,Chen, Hong,Hu, Linyong,et al. miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(7):760-774. |
| APA | Fang, Xingtang.,Chen, Hong.,Hu, Linyong.,Zhang, Chunlei.,Wang, Yanhong.,...&Wang, Jian.(2018).miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(7),760-774. |
| MLA | Fang, Xingtang,et al."miR-487b-3p Suppresses the Proliferation and Differentiation of Myoblasts by Targeting IRS1 in Skeletal Muscle Myogenesis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.7(2018):760-774. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。