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In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media

文献类型：期刊论文

作者Zhu, Changhui1,2,4; Guo, Feng1; Guo, Xiaoqian1,3,4; Li, Xingkang4
刊名BIORESOURCES
出版日期2016-11-01
卷号11期号:4页码:9068-9078
关键词In Situ Saccharification Aqueous-ionic Liquid Media Cellulase Cellobiase Synergism
ISSN号1930-2126
DOI10.15376/biores.11.4.9068-9078
文献子类Article
英文摘要In situ enzymatic saccharification is one promising approach to efficiently convert non-food biomass to glucose as a platform chemical. The goal of the present research was to illustrate the catalytic behaviors and deactivation mechanisms of cellulase and cellobiase in an aqueous-ionic liquid (IL) media. The hydrolysis reaction rate was considerably increased because of the increased porosity and reduced crystallinity of the substrate. To lower the inhibitory effect of accumulated cellobiose on cellulase, a multi-enzyme synergetic system was explored. Compared with the single cellulase saccharification, addition of cellobiase resulted in a substantial increase in total reducing sugars (TRS) yield (88.0% vs. 59.1%) and glucose yield (40.6% vs. 28.9%) when the activity unit ratio of cellobiase/cellulase was 0.95. Cellulase showed a higher stability in aqueous 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) media. However, the activity of cellobiase quickly decreased, by 66.5%, after 6 h of incubation.
WOS关键词LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; PRETREATMENT ; DISSOLUTION ; SOLVATION ; MECHANISM ; FEATURES
语种英语
WOS记录号WOS:000391801300071
出版者NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
资助机构Chinese Academy of Sciences(Y2XB021K01) ; National Natural Science Foundation of China(31270620)
源URL[http://ir.giec.ac.cn/handle/344007/14022]  
专题中国科学院广州能源研究所
通讯作者Guo, Feng
作者单位1.Dalian Univ Technol, Sch Food & Environm, Panjin Campus,2 Dagong Rd, Panjin 124221, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Plant Funct Genom Lab, 88 Xuefu Rd, Kunming 650223, Peoples R China
4.Chinese Acad Sci, Grad Univ, 19A Yuqun Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,et al. In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media[J]. BIORESOURCES,2016,11(4):9068-9078.
APA Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,&Li, Xingkang.(2016).In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media.BIORESOURCES,11(4),9068-9078.
MLA Zhu, Changhui,et al."In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media".BIORESOURCES 11.4(2016):9068-9078.

入库方式： OAI收割

来源：广州能源研究所

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