INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION
文献类型：期刊论文
| 作者 | Liang Cuiyi ; Xu Huijuan; Zhang Yu; Guo Ying; Chen Xiaoyan; Yuan Zhenhong; He Minchao; Xu Jingliang
|
| 刊名 | CELLULOSE CHEMISTRY AND TECHNOLOGY
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 51期号:7-8页码:719-729 |
| 关键词 | Beta-glucosidase Induction Biomass Materials Enzyme Biocatalysis Bioconversion Ethanol Fermentation |
| 文献子类 | Article |
| 英文摘要 | To obtain highly active beta-glucosidase, low value agricultural residues were used to induce enzyme production. The results showed that rice bran was the best substrate to induce beta-glucosidase, likely due to its high magnesium, potassium, and calcium content. The optimum temperature and pH of beta-glucosidase were 65 degrees C and 4.8, respectively. This enzyme was thermo-tolerant and fairly stable, with a long half-life of 15 days at 50 degrees C. In addition, the enzyme activity was best stimulated in a concentration of 10% (v/v) ethanol. The effects of beta-glucosidase on fed-batch hydrolysis of cellulose and high-temperature simultaneous saccharification and fermentation (SSF) were examined. With a cellulase loading of 10 FPU/g of substrate and a beta-glucosidase loading of 15 IU/g of substrate, the final substrate loading of 35% dry mass, sugar concentration could attain 225.84 g/L after 120 h of hydrolysis. When the fed-batch SSF process was performed with Kluyveromyces marxianus NCYC 587 operated at 42 degrees C, after 72 h of fermentation, the maximum ethanol concentration was approximately 49.07 g/L, indicating that beta-glucosidase was suitable for lignocellulose conversion into ethanol. |
| WOS关键词 | SIMULTANEOUS SACCHARIFICATION ; BIOETHANOL PRODUCTION ; KLUYVEROMYCES-MARXIANUS ; TRICHODERMA-REESEI ; ETHANOL-PRODUCTION ; CELLULASE ; PURIFICATION ; IMPROVEMENT ; SELECTION ; RESIDUES |
| 语种 | 英语 |
| WOS记录号 | WOS:000413720100014 |
| 资助机构 | Science & Technology Project of Guangdong Province(2017 A010105018) ; National High Technology Research and Development Program of China (863 Program)(2015AA020202) ; Bureau of International Cooperation, Chinese Academy of Sciences(182344KYSB20160056) ; Program of National Natural Science Foundation of China(21506215 ; 51606203) |
| 源URL | [http://ir.giec.ac.cn/handle/344007/15860]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou Inst Energy Convers, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang Cuiyi,Xu Huijuan,Zhang Yu,et al. INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION[J]. CELLULOSE CHEMISTRY AND TECHNOLOGY,2017,51(7-8):719-729. |
| APA | Liang Cuiyi.,Xu Huijuan.,Zhang Yu.,Guo Ying.,Chen Xiaoyan.,...&Xu Jingliang.(2017).INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION.CELLULOSE CHEMISTRY AND TECHNOLOGY,51(7-8),719-729. |
| MLA | Liang Cuiyi,et al."INDUCTION OF HIGHLY ACTIVE beta-GLUCOSIDASE FROM BIOMASS MATERIALS AND ITS APPLICATION ON LIGNOCELLULOSE HYDROLYSIS AND FERMENTATION".CELLULOSE CHEMISTRY AND TECHNOLOGY 51.7-8(2017):719-729. |
入库方式： OAI收割
来源：广州能源研究所
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