Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva
文献类型：期刊论文
| 作者 | Shang, Changhua1 ; Zhu, Shunni1; Qin, Lei1; Alam, Mohammad A.1; Wang, Zhongming1; Yuan, Zhenhong1,2; Xie, Jun3
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| 刊名 | JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
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| 出版日期 | 2017-10-01 |
| 卷号 | 11期号:5页码:483-490 |
| 关键词 | Differential Expression Dunaliella parva Lipase Lipid Degradation Nitrogen Limitation |
| ISSN号 | 1556-6560 |
| DOI | 10.1166/jbmb.2017.1691 |
| 通讯作者 | Yuan, Zhenhong(yuanzh@ms.giec.ac.cn) |
| 英文摘要 | Dunaliella parva grew in different nitrogen concentrations, which were associated with lipid accumulation. D. parva could accumulate lipids. However, the underlying mechanism of lipid degradation requires further research. Thus, it is important to analyze lipid degradation to elucidate lipid accumulation. In this report, a gene encoding lipase from the green alga D. parva was isolated and characterized. Experimental results showed that the full-length cDNA of lipase gene was 4,583 bp, with an open reading frame of 3,183 bp, a 73 bp 5'-untranslated region and a 1,327 bp 3'-untranslated region. Potential regulatory elements related to hormones, light and unfavorable environments were found in the 1,828 bp 5'-flanking region. Analysis of lipase gene expression showed a correlation between lipase gene expression and nitrogen concentrations, which indicated that lipase gene may be responsive to nitrogen limitation stress. The cloning and expression analysis of lipase gene provided a basis for studying the regulatory mechanism of lipid degradation and the massive accumulation of lipids or lipase in D. parva. |
| WOS关键词 | LED LIGHT WAVELENGTHS ; DOMAIN TRIACYLGLYCEROL LIPASE ; INTENSITY SUPPLY STRATEGIES ; MICROALGAE CHLORELLA-SP ; CHLAMYDOMONAS-REINHARDTII ; BIODIESEL PRODUCTION ; NUTRIENT REDUCTION ; BIOGAS UPGRADE ; PURIFICATION ; PERFORMANCE |
| 资助项目 | Natural Science Foundation of Guangdong Province[2016A030313171] ; Natural Science Foundation of Guangdong Province[2016A030312007] ; National Natural Sciences Foundation of China[51476177] ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences[y507j21001] |
| WOS研究方向 | Chemistry ; Energy & Fuels ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000416103100013 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 资助机构 | Natural Science Foundation of Guangdong Province ; National Natural Sciences Foundation of China ; Foundation of Key Laboratory of Renewable Energy, Chinese Academy of Sciences |
| 源URL | [http://ir.giec.ac.cn/handle/344007/16775]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Yuan, Zhenhong |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Renewable Energy, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China 3.South China Agr Univ, Inst New Energy & New Mat, Key Lab Energy Plants Resource & Utilizat, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shang, Changhua,Zhu, Shunni,Qin, Lei,et al. Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2017,11(5):483-490. |
| APA | Shang, Changhua.,Zhu, Shunni.,Qin, Lei.,Alam, Mohammad A..,Wang, Zhongming.,...&Xie, Jun.(2017).Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,11(5),483-490. |
| MLA | Shang, Changhua,et al."Cloning and Expression Analysis of a Lipase Gene from Dunaliella parva".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 11.5(2017):483-490. |
入库方式： OAI收割
来源：广州能源研究所
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