We investigate a new type of molybdenum disulfide (MoS2)-doped sol-gel glass saturable absorber (SA) fabricated by sol-gel technique. The reagents used for the sol-gel glass contain Tetraethyl orthosilicate (TEOS), ethanol, water, and hydrochloric acid. Different from the traditional ways of fabricating SAs, the MoS2 in our method is encapsulated by inorganic sol-gel glass instead of polymer compound with low laser damage resistance, which greatly increases the optical damage threshold of MoS2 SA. The MoS2-doped sol-gel glass as an SA is experimentally demonstrated in a passively mode-locked ytterbium-doped fiber laser (YDFL). Stable mode-locked pulse trains are successfully generated in the normal dispersion regime with a pulse width of 13.8 ps and the average output power of 34.6 mW. The fluctuation of the central wavelength and spectral bandwidth is as low as 0.9% in one week, which indicates that the mode-locking state has good environmental stability. To the best of our knowledge, it is the first example of sol-gel glass SA for ultrafast pulses generated in YDFL, which potentially gives a new approach to improve optical damage threshold and long-term working stability for broadband absorbers. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement