Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction
文献类型：期刊论文
| 作者 | Sajjad, Wasim1,2; Zheng, Guodong1; Ma, Xiangxian1; Rafiq, Muhammad3; Irfan, Muhammad4,5; Xu, Wang1,2; Ali, Barkat2,6 |
| 刊名 | JOURNAL OF BASIC MICROBIOLOGY
 |
| 出版日期 | 2019-03-01 |
| 卷号 | 59期号:3页码:323-336 |
| ISSN号 | 0233-111X |
| 关键词 | Acidithiobacillus ferrooxidans Baiyin Copper Mine bioleaching Leptospirillum ferriphilum Leptospirillum ferrooxidans |
| DOI | 10.1002/jobm.201800433 |
| 英文摘要 | The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous iron-oxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod. The optimum growth pH and temperature for all isolates were 1.5 and 30 degrees C, respectively, and showed extreme acidophilic nature. All the isolates showed obligate chemoautotrophic nature and used ferrous iron and pyrite as an energy source, however, isolates WG102 and WG103 were unable to use sulfur, while isolate WG101 could use elemental sulfur and reduced inorganic sodium thiosulfate as an energy source. The phylogenetic analyses based on 16S rRNA sequences revealed that the isolates WG101, WG102, and WG103 were homologous with Acidithiobacillus ferrooxidans strain AS2 (99%), Leptospirillum ferriphilum strain YSK (98%), and Leptospirillum ferrooxidans strain L15 (98%), respectively. These bacterial isolates showed efficient copper and zinc dissolution from the ore. The metals dissolution rate of At. ferrooxidans strain WG101 was 54.5 +/- 4.33% (copper) and 49.6 +/- 5% (zinc). The metals recovery rate of L. ferriphilum strain WG102 was 45.7 +/- 3.5% (copper) and 40.5 +/- 2.5% (zinc). The recovery rate of copper and zinc was 49.6 +/- 4% and 46.5 +/- 3% respectively in the case of L. ferrooxidans strain WG103. The findings of this study are consistent with the notion that the indigenous bacteria are more efficient in minerals dissolution. |
| WOS关键词 | PRINTED-CIRCUIT BOARDS ; ACIDITHIOBACILLUS-FERROOXIDANS ; LEPTOSPIRILLUM-FERRIPHILUM ; MIXED CULTURE ; FERROUS IRON ; SOLID MEDIUM ; DRAINAGE ; STRAIN ; OXIDATION ; IDENTIFICATION |
| 资助项目 | CAS-TWAS President's Fellowship ; Natural Science Foundation of China[41572352] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000461581400010 |
| 资助机构 | CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; CAS-TWAS President's Fellowship ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/90894]  |
| 专题 | 地质与地球物理研究所_兰州油气中心 |
| 通讯作者 | Zheng, Guodong |
| 作者单位 | 1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Petr Resources, Gansu Prov Key Lab Petr Resources Res, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Bristol, Sch Geog Sci, Bristol Glaciol Ctr, Fac Sci, Bristol, Avon, England 4.Univ Florida, Dept Microbiol & Cell Sci, Genet Inst, Gainesville, FL 32611 USA 5.Univ Florida, Inst Food & Agr Sci, Gainesville, FL 32611 USA 6.Univ Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryosphere Sci, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sajjad, Wasim,Zheng, Guodong,Ma, Xiangxian,et al. Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction[J]. JOURNAL OF BASIC MICROBIOLOGY,2019,59(3):323-336. |
| APA | Sajjad, Wasim.,Zheng, Guodong.,Ma, Xiangxian.,Rafiq, Muhammad.,Irfan, Muhammad.,...&Ali, Barkat.(2019).Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction.JOURNAL OF BASIC MICROBIOLOGY,59(3),323-336. |
| MLA | Sajjad, Wasim,et al."Culture-dependent hunt and characterization of iron-oxidizing bacteria in Baiyin Copper Mine, China, and their application in metals extraction".JOURNAL OF BASIC MICROBIOLOGY 59.3(2019):323-336. |
入库方式： OAI收割
来源：地质与地球物理研究所
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