Investigation of Interaction Between Two Neutralizing Monoclonal Antibodies and SARS Virus Using Biosensor Based on Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Qi C(齐财); Duan JZ; Wang ZH(王战会); Chen YY; Zhang PH; Zhan L ; Yan XY; Cao WC; Jin G(靳刚)
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| 刊名 | Biomedical Microdevices
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| 出版日期 | 2006 |
| 卷号 | 8期号:3页码:247-253 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| ISSN号 | 1387-2176 |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100080, Peoples R China. |
| 中文摘要 | Two neutralizing human scFv, b1 and h12 were identified initially using ELISA, employing highly purified virus as the coating antigen. The biosensor technique based on imaging ellipsometrywas employed directly to detect two neutralizing monoclonal antibodies and serial serum samples from 10SARSpatients and 12 volunteers who had not SARS. Further, the kinetic process of interaction between the antibodies and SARS-CoV was studied using the real-time function of the biosensor. The biosensor is consistent with ELISA that the antibody h12 showed a higher affinity in encountering the virus than antibody b1. The affinity of antibody b1 and antibody h12 was 9.5×106 M−1 and 1.36×107 M−1, respectively. As a label free method, the biosensor based on imaging ellipsometry proved to be a more competent mechanism for measuring serum samples from SARS patients and the affinity between these antibodies and the SARS coronavirus. |
| 学科主题 | 力学 |
| 类目[WOS] | Engineering, Biomedical ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Engineering ; Science & Technology - Other Topics |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; CORONAVIRUS SPIKE PROTEIN ; REAL-TIME ANALYSIS ; REACTION-KINETICS ; COV ; PCR ; CORTICOSTEROIDS ; VISUALIZATION ; SYSTEM ; SERUM |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239664600008 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/15534]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Qi C,Duan JZ,Wang ZH,et al. Investigation of Interaction Between Two Neutralizing Monoclonal Antibodies and SARS Virus Using Biosensor Based on Imaging Ellipsometry[J]. Biomedical Microdevices,2006,8(3):247-253. |
| APA | 齐财.,Duan JZ.,王战会.,Chen YY.,Zhang PH.,...&靳刚.(2006).Investigation of Interaction Between Two Neutralizing Monoclonal Antibodies and SARS Virus Using Biosensor Based on Imaging Ellipsometry.Biomedical Microdevices,8(3),247-253. |
| MLA | 齐财,et al."Investigation of Interaction Between Two Neutralizing Monoclonal Antibodies and SARS Virus Using Biosensor Based on Imaging Ellipsometry".Biomedical Microdevices 8.3(2006):247-253. |
入库方式： OAI收割
来源：力学研究所
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