齿肋赤藓四个 ScDREBs 基因特性及抗逆功能分析
文献类型：学位论文
| 作者 | 梁玉青 |
| 答辩日期 | 2017-05-01 |
| 文献子类 | 硕士 |
| 授予单位 | 中国科学院大学 |
| 授予地点 | 新疆乌鲁木齐 |
| 导师 | 管开云 ; 张道远 |
| 关键词 | DREB转录因子 齿肋赤藓 胁迫抗性 功能验证 DREB transcription factor Syntrichia caninervis stress tolerance functional verification |
| 学位专业 | 理学硕士 |
| 英文摘要 | DREB transcription factor, belonging to the AP2 / EREBP family, is specificallypresent in plants and can regulate the expression of a series of downstream genes related todrought, salinity, low temperature and high temperature stresses, which plays vital roles inresponse to external adverse environmental factors, and are extensively investigated invarious plants. Syntrichia caninervis Mitt., an extremely dessication tolerance moss, isconsidered as a good candidate species for gene mining because of its outstanding drought,heat and UV resistant tolerance. In the past research, we build dehydration-rehydrationtranscriptome profile of S. caninervis and screened DREB family genes, and analyzed ongene expression pattern and gene abiotic function by yeast system of ten ScDREBs. In thisstudy, we selected four potential key ScDREBs genes and delve on further stress tolerancefunctional in Arabdopsis system. The work will shed light on the extremely stress tolerancemechanism of the moss, and will benefit for further development and utilization of thegene resources in crop breeding. The main conclusions are as follows:(1) Subcellular location assay of four ScDREBs proteins showed that all the fourScDREBs were located in both the nucleus and the cytoplasm of the onion or tobaccocells;(2) Transactivation activity assay of four ScDREBs proteins showed that ScDREB10,ScDREB3and ScDREB6 had transactivation activity except that ScDREB8 showed notransactivation activity;(3) Four ScDREBs genes were stably transformed into Arabidopsis by floral dipmethod, and the positive T3 generations were obtained for further gene function validation.Under the drought, salt, cold and heat treatment, the phenotypic change (root length andfresh weight) were observed, the physiological response (ROS level, determination ofantioxidant enzymes and proline content) were monitored and downstream gene expressionpattern were detected. The results showed that:Under the drought, salt and heat treatment, the Overexpressing-ScDREB10 lines had significantly higher seed germination rate and seedlings survival rate of transgenic linesthan wild type (WT). The transgenic lines also showed lower ROS level and higher SODand POD activities than those of WT at seedling stage. In addition, the expression ofScDREB10 gene could positively regulated a series of downstream stress toleranceassociated genes expression, which indicated that overexpressing-ScDREB10 increasedrought and salt tolerance in Arabidopsis; Under the salt treatment, the germination rate and seedlings survival rate ofoverexpressing-ScDREB8 gene lines were significantly higher than WT. The level of ROSin transgenic lines was significantly lower than that in WT, and the activity of antioxidantenzyme (SOD、POD) and proline content were significantly higher at seedling stage. Inaddition, ScDREB8 positively regulated a series of downstream stress tolerance associatedgenes expression. All results indicated that ScDREB8 increased salt tolerance inArabidopsis; Under the drought, high salt, cold and heat treatment, the overexpressing-ScDREB3lines had no significantly higher seed germination rate than the WT, but the survival ratewas significantly higher than that of the wild type under the extreme cold treatment(-20 ℃)at the seedling stage, showing that overexpressing-ScDREB3 gene might increase coldtolerance in Arabidopsis;Under the drought, high salt treatment, ScDREB6 transgenic lines showed nosignificant differences compared with the wild type, indicating that ScDREB6 might haveno abiotic stress tolerance ability. While the transgenic Arabidopsis showed significantlonger hypocotyl and a faster growth cycle phenotype at the seedling stage, implyingstrong hypocotyl elongation and growth-promoting function. |
| 学科主题 | 植物学 |
| 语种 | 中文 |
| 源URL | [http://ir.xjlas.org/handle/365004/14885]  |
| 专题 | 新疆生态与地理研究所_研究系统_荒漠环境研究室 |
| 作者单位 | 中国科学院新疆生态与地理研究所 |
| 推荐引用方式 GB/T 7714 | 梁玉青. 齿肋赤藓四个 ScDREBs 基因特性及抗逆功能分析[D]. 新疆乌鲁木齐. 中国科学院大学. 2017. |
入库方式： OAI收割
来源：新疆生态与地理研究所
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