新疆地区棉铃虫的抗性监测及其交配选择性研究
文献类型：学位论文
| 作者 | 王盼盼 |
| 答辩日期 | 2017-05-01 |
| 文献子类 | 硕士 |
| 授予单位 | 中国科学院大学 |
| 授予地点 | 新疆乌鲁木齐 |
| 导师 | 吕昭智 |
| 关键词 | Bt棉花 棉铃虫 抗性 寄主来源 交配 Bt-cotton Helicoverpa armigera resistance host mating |
| 学位专业 | 工程硕士 |
| 英文摘要 | Bt-cotton controls Helicoverpa armigera effectively. Single toxin (Cry1Ac) Bt-cottonhas been intensively planted for nearly 15 years in Xinjiang Province, but it is not endorsedby any authority. The status of Cry1Ac susceptibility of H. armigera in this region has notbeen routinely monitored. The same is true for the susceptibility of H. armigera to cottonexpressing Cry2Ab toxin, which has not been released in China. The susceptibility of H.armigera field populations to Cry1Ac and Cry2Ab was assessed in nine populations ofXinjiang in 2014–2015. In order to learn the main host of larva and mating of H. armigera,moths collected from 9 populations were dissected and analyzed by stable carbon isotopein 2014-2015. Moreover, the mating experiment of H.armigera between different resistantlevels and ages was completed in lab from August to December in 2016. The main resultsare as follows:1. The response to Cry1Ac of the nine H. armigera strains ranged from 3.16 to 16.94μg/ml for LC50, 0.013 to 0.741 μg/ml for IC50, and <0.001 to 0.038 μg/ml for EC50 overtwo consecutive years. The susceptibility of H. armigera field populations to Cry1Ac inXinjiang does not appear to have shifted significantly over the past 15 years sinceBt-cotton has been intensive planted. There was no significant difference in susceptibilityto Cry1Ac between strains originating from collections on small-holder versus broad-acrefarms.2. The susceptibility of these strains to Cry2Ab ranged from 3.43 to 19.05 μg/ml for LC50,0.16 to 3.81 μg/ml for IC50, and <0.001 to 0.24 μg/ml for EC50 over two years. There wasno significant difference in susceptibility to Cry2Ab between strains originating fromcollections on small-holder versus broad-acre farms. Pearson correlation analysis was usedto estimate the correlation between Cry1Ac and Cry2Ab responses. Across the nine fieldpopulations, there were no significant correlations between Cry1Ac and Cry2Absusceptibility for any of the three measurements in 2014 or in 2015.3. The host use by H. armigera larvae. The results showed that in different months,female and male larvae had parasitise on different hosts. However, most of them feed on C3 hosts.For Korle, there were 85% female from C3 and 90% male from C3 in 2013, 85%female from C3 and 83.5% male from C3 in 2014, 58% female from C3 and 56% malefrom C3.The mating performance of H.armigerea in Korla. There were always some moths notmating in the field. Mostly, high mating proporations had high mating frenquencies. Basedon stable carbon isotope analysis, in 2013 and 2014, the mating percentage of C3*C4 wereabout 55% and 54% in June, 8% and 9.5% in July, 22% and 28% in August and after,respectively. The mating percentage of C3*C4 was 41% in 2015. The high percentage ofC3*C4 indicated that the H. armigera can mate randomly from different hosts and C4could be a host for H.armigera.4. Mating. The results of multi-choice mating experiment, single-choice matingexperiment and single pairs mating experiment showed there were no significant differenceof the mating ratio, mating frequency and duration time between mating types. Theseindicated that H.armigera between different resistant levels and ages could mate randomly.There were significant difference for the mating between 5-day old male and 1-day oldmale. This indicated that the males’ age could affect their mating choice. |
| 学科主题 | 生物工程 |
| 语种 | 中文 |
| 源URL | [http://ir.xjlas.org/handle/365004/14900]  |
| 专题 | 新疆生态与地理研究所_研究系统_荒漠环境研究室 |
| 作者单位 | 中国科学院新疆生态与地理研究所 |
| 推荐引用方式 GB/T 7714 | 王盼盼. 新疆地区棉铃虫的抗性监测及其交配选择性研究[D]. 新疆乌鲁木齐. 中国科学院大学. 2017. |
入库方式： OAI收割
来源：新疆生态与地理研究所
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