A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces
文献类型：期刊论文
| 作者 | Keke SHI; Qi ZENG; Honghong SONG; Jinghan SONG; Xiaowei SONG |
| 刊名 | Asian Herpetological Research
 |
| 出版日期 | 2018-03 |
| 卷号 | 9期号:1页码:1-12 |
| 关键词 | Bufo gargarizans DNA extraction intestinal microflora Phenol-chloroform 16S rDNA |
| DOI | 10.16373/j.cnki.ahr.170062 |
| 英文摘要 | The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in drawing the gut microbiota profiles, the integrative analysis of gut microbiota produced by different studies must be performed with circumspection. In this study, we compared the efficacy of two DNA extraction methods (i.e., a phenol-chloroform method and TIANamp Stool DNA Kit) in describing intestinal and fecal bacterial communities of transplanted Asiatic toad (Bufo gargarizans) tadpoles. In terms of the DNA extraction quality (i.e., DNA purity and yield rate) and the consistency in between fecal and intestinal microbiota structures (i.e., α and β diversity indices), the phenol-chloroform method was more robust than this commercial stool kit in profiling gut microbiota of tadpoles with feces. |
| 语种 | 英语 |
| 源URL | [http://210.75.237.14/handle/351003/29574]  |
| 专题 | 《亚洲两栖爬行动物研究》_2018_Vol.9, No.1 |
| 通讯作者 | Xiaowei SONG |
| 推荐引用方式 GB/T 7714 | Keke SHI,Qi ZENG,Honghong SONG,et al. A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces[J]. Asian Herpetological Research ,2018,9(1):1-12. |
| APA | Keke SHI,Qi ZENG,Honghong SONG,Jinghan SONG,&Xiaowei SONG.(2018).A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces.Asian Herpetological Research ,9(1),1-12. |
| MLA | Keke SHI,et al."A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces".Asian Herpetological Research 9.1(2018):1-12. |
入库方式： OAI收割
来源：成都生物研究所
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