中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats

文献类型:期刊论文

作者Yuan, Qingjie1; Li, Yang2; Deng, Xiaofei3; Shi, Huawei2; Zhao, Zhenwu2; Wang, Chunye4; Feng, Xuegong1; Guo, Jianyou3; Guo, Rongjuan4
刊名EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
出版日期2019
页码12
ISSN号1741-427X
DOI10.1155/2019/6054926
文献子类Article
英文摘要

Objective. To clarify the effectiveness and mechanism of the Chinese herbal formula Xingpi Kaiyu Fang (XPKYF) which is composed of American ginseng (Xi-Yang-shen), Radix curcumae (Yu-Jin), Acori tatarinowii rhizoma (Shi-Chang-pu), and Hypericum perforatum (Guan-Ye-lian-qiao) in depressed rats. Methods. The rat model of depression was established by chronic unpredictable mild stress (CUMS) method for 6 weeks. Rats were randomly divided into six groups: control group, CUMS group, CUMS+XPKYF (3.6g/kg/d, 7.2g/kg/d, 14.4g/kg/d) groups, and CUMS+sertraline (4.5mg/kg/d) group. The sucrose preference test and the forced swimming test were performed to assess the rats' depression behavior. Mitochondrial ultrastructure was observed by transmission electron microscope and adenosine triphosphate (ATP) content, sodium potassium ATPase (Na/K-ATPase) activity, and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day. Results. Rats subjected to six weeks of CUMS exhibited decreased sucrose preference ratio and prolonged immobility time. CUMS reduced ATP content in hippocampus, decreased Na/K-ATPase activity and respiratory chain complex I, III, and IV activities in hippocampus and gastrocnemius muscle, and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle. XPKYF at 14.4g/kg, the efficacy trend of which was better than the other drug groups, could prevent the stress-induced depressed behavior changes, inhibit the decrease of Na/K-ATPase activity in hippocampus, inhibit the decrease of respiratory chain complex III activities in hippocampus and gastrocnemius muscle, and protect mitochondria from ultrastructural damage. Conclusions. Energy deficiency and damaged mitochondrial ultrastructure were found in hippocampus and gastrocnemius muscle of depressed rats established by CUMS. XPKYF could partly reverse alterations in ATP, Na/K-ATPase, and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage. This provides another experimental evidence for the clinical application of XPKYF in the treatment of depression.

WOS关键词na+/k+-atpase ; Mitochondrial ; Behavior ; Models ; Brain
资助项目National Natural Science Foundation of China[81573843] ; CAS Key Laboratory of Mental Health, Institute of Psychology[KLMH2018K03]
WOS研究方向Integrative & Complementary Medicine
语种英语
出版者HINDAWI LTD
WOS记录号WOS:000455757900001
源URL[http://ir.psych.ac.cn/handle/311026/28090]  
专题心理研究所_中国科学院心理健康重点实验室
通讯作者Guo, Jianyou; Guo, Rongjuan
作者单位1.Beijing Hosp Integrated Tradit Chinese & Western, Beijing 100039, Peoples R China
2.Beijing Univ Chinese Med, Beijing 100029, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Mental Hlth, Inst Psychol, Beijing 100101, Peoples R China
4.Beijing Univ Chinese Med, Dongfang Hosp, Beijing 100078, Peoples R China
推荐引用方式
GB/T 7714
Yuan, Qingjie,Li, Yang,Deng, Xiaofei,et al. Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2019:12.
APA Yuan, Qingjie.,Li, Yang.,Deng, Xiaofei.,Shi, Huawei.,Zhao, Zhenwu.,...&Guo, Rongjuan.(2019).Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,12.
MLA Yuan, Qingjie,et al."Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2019):12.

入库方式: OAI收割

来源:心理研究所

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