Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats
文献类型:期刊论文
作者 | Yuan, Qingjie1; Li, Yang2; Deng, Xiaofei3; Shi, Huawei2; Zhao, Zhenwu2; Wang, Chunye4; Feng, Xuegong1; Guo, Jianyou3; Guo, Rongjuan4 |
刊名 | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE |
出版日期 | 2019 |
页码 | 12 |
ISSN号 | 1741-427X |
DOI | 10.1155/2019/6054926 |
文献子类 | Article |
英文摘要 | Objective. To clarify the effectiveness and mechanism of the Chinese herbal formula Xingpi Kaiyu Fang (XPKYF) which is composed of American ginseng (Xi-Yang-shen), Radix curcumae (Yu-Jin), Acori tatarinowii rhizoma (Shi-Chang-pu), and Hypericum perforatum (Guan-Ye-lian-qiao) in depressed rats. Methods. The rat model of depression was established by chronic unpredictable mild stress (CUMS) method for 6 weeks. Rats were randomly divided into six groups: control group, CUMS group, CUMS+XPKYF (3.6g/kg/d, 7.2g/kg/d, 14.4g/kg/d) groups, and CUMS+sertraline (4.5mg/kg/d) group. The sucrose preference test and the forced swimming test were performed to assess the rats' depression behavior. Mitochondrial ultrastructure was observed by transmission electron microscope and adenosine triphosphate (ATP) content, sodium potassium ATPase (Na/K-ATPase) activity, and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day. Results. Rats subjected to six weeks of CUMS exhibited decreased sucrose preference ratio and prolonged immobility time. CUMS reduced ATP content in hippocampus, decreased Na/K-ATPase activity and respiratory chain complex I, III, and IV activities in hippocampus and gastrocnemius muscle, and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle. XPKYF at 14.4g/kg, the efficacy trend of which was better than the other drug groups, could prevent the stress-induced depressed behavior changes, inhibit the decrease of Na/K-ATPase activity in hippocampus, inhibit the decrease of respiratory chain complex III activities in hippocampus and gastrocnemius muscle, and protect mitochondria from ultrastructural damage. Conclusions. Energy deficiency and damaged mitochondrial ultrastructure were found in hippocampus and gastrocnemius muscle of depressed rats established by CUMS. XPKYF could partly reverse alterations in ATP, Na/K-ATPase, and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage. This provides another experimental evidence for the clinical application of XPKYF in the treatment of depression. |
WOS关键词 | na+/k+-atpase ; Mitochondrial ; Behavior ; Models ; Brain |
资助项目 | National Natural Science Foundation of China[81573843] ; CAS Key Laboratory of Mental Health, Institute of Psychology[KLMH2018K03] |
WOS研究方向 | Integrative & Complementary Medicine |
语种 | 英语 |
出版者 | HINDAWI LTD |
WOS记录号 | WOS:000455757900001 |
源URL | [http://ir.psych.ac.cn/handle/311026/28090] |
专题 | 心理研究所_中国科学院心理健康重点实验室 |
通讯作者 | Guo, Jianyou; Guo, Rongjuan |
作者单位 | 1.Beijing Hosp Integrated Tradit Chinese & Western, Beijing 100039, Peoples R China 2.Beijing Univ Chinese Med, Beijing 100029, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Mental Hlth, Inst Psychol, Beijing 100101, Peoples R China 4.Beijing Univ Chinese Med, Dongfang Hosp, Beijing 100078, Peoples R China |
推荐引用方式 GB/T 7714 | Yuan, Qingjie,Li, Yang,Deng, Xiaofei,et al. Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2019:12. |
APA | Yuan, Qingjie.,Li, Yang.,Deng, Xiaofei.,Shi, Huawei.,Zhao, Zhenwu.,...&Guo, Rongjuan.(2019).Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,12. |
MLA | Yuan, Qingjie,et al."Effects of Xingpi Kaiyu Fang on ATP, Na/K-ATPase, and Respiratory Chain Complexes of Hippocampus and Gastrocnemius Muscle in Depressed Rats".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2019):12. |
入库方式: OAI收割
来源:心理研究所
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