Aptamer based surface plasmon resonance sensor for aflatoxin B1
文献类型：期刊论文
| 作者 | Sun, Linlin; Wu, Liqing; Zhao, Qiang |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2017-02-10 |
| 卷号 | 184期号:8页码:2605-2610 |
| 关键词 | Mycotoxin Spr Direct Format Assay Affinity Binding Antibody Chip Regeneration Immobilization Dissociation Stability |
| 英文摘要 | The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39249]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Linlin,Wu, Liqing,Zhao, Qiang. Aptamer based surface plasmon resonance sensor for aflatoxin B1[J]. MICROCHIMICA ACTA,2017,184(8):2605-2610. |
| APA | Sun, Linlin,Wu, Liqing,&Zhao, Qiang.(2017).Aptamer based surface plasmon resonance sensor for aflatoxin B1.MICROCHIMICA ACTA,184(8),2605-2610. |
| MLA | Sun, Linlin,et al."Aptamer based surface plasmon resonance sensor for aflatoxin B1".MICROCHIMICA ACTA 184.8(2017):2605-2610. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。