CDC42 expression is altered by dioxin exposure and mediated by multilevel regulations via AhR in human neuroblastoma cells
文献类型：期刊论文
| 作者 | Xu, Tuan; Xie, Heidi Q.; Li, Yunping; Xia, Yingjie; Chen, Yangsheng; Xu, Li; Wang, Lingyun; Zhao, Bin |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 7期号:0页码:- |
| 英文摘要 | Emerging evidence has shown that dioxin causes dysregulation of microRNAs (miRs) in a variety of tissues or cells. However, little is known about dioxin effects on neuronal miRs expression. In the present study, 277 differentially expressed miRs were identified by miRs microarray analysis in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, at 10(-10) M) treated SK-N-SH neuroblastoma cells. Among them, 53 miRs exhibited changes of more than 0.4-fold. Consistent with the microarray data, we verified the induction effect of TCDD on hsa-miR-608 expression, which is a primate-specific miR associated with brain functions. Bioinformatics analysis showed involvement of hsa-miR-608 in cytoskeleton organization, in which one of the hsa-miR-608 target genes, Cell Division Cycle 42 (CDC42), might play a role. We also confirmed induction of CDC42 expression by TCDD in SK-N-SH cells. TCDD induced the expression of CDC42 mRNA in hsa-miR-608 inhibitor transfected cells more obviously than in control cells, suggesting involvement of both transcriptional and post-transcriptional mechanisms in the TCDD-induced CDC42 regulation. Furthermore, CH223191, an antagonist of the aryl hydrocarbon receptor (AhR), counteracted TCDD-induced hsa-miR-608 and CDC42 expression. These results indicated that AhR not only mediates transcriptional induction of CDC42, but also hsa-miR-608-induced post-transcriptional regulation of CDC42 in dioxin treated neuroblastoma cells. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39264]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu, Tuan,Xie, Heidi Q.,Li, Yunping,et al. CDC42 expression is altered by dioxin exposure and mediated by multilevel regulations via AhR in human neuroblastoma cells[J]. SCIENTIFIC REPORTS,2017,7(0):-. |
| APA | Xu, Tuan.,Xie, Heidi Q..,Li, Yunping.,Xia, Yingjie.,Chen, Yangsheng.,...&Zhao, Bin.(2017).CDC42 expression is altered by dioxin exposure and mediated by multilevel regulations via AhR in human neuroblastoma cells.SCIENTIFIC REPORTS,7(0),-. |
| MLA | Xu, Tuan,et al."CDC42 expression is altered by dioxin exposure and mediated by multilevel regulations via AhR in human neuroblastoma cells".SCIENTIFIC REPORTS 7.0(2017):-. |
入库方式： OAI收割
来源：生态环境研究中心
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