Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells
文献类型：期刊论文
| 作者 | Guo, Liang-Hong; Cui, Xuejing; Wan, Bin; Yang, Yu; Ren, Xiaomin |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-12-13 |
| 卷号 | 7期号:0页码:- |
| 英文摘要 | Cellular uptake and exocytosis of SWCNTs are fundamental processes determining their intracellular concentration and effects. Despite the great potential of acid-oxidized SWCNTs in biomedical field, understanding of the influencing factors on these processes needs to be deepened. Here, we quantitatively investigated uptake and exocytosis of SWCNTs in three lengths-630 (+/- 171) nm (L-SWCNTs), 390 (+/- 50) nm (M-SWCNTs), and 195 (+/- 63) nm (S-MWCNTs) in macrophages. The results showed that the cellular accumulation of SWCNTs was a length-independent process and nonmonotonic in time, with the most SWCNTs (3950 fg/cell) accumulated at 8 h and then intracellular SWCNTs dropped obviously with time. The uptake rate of SWCNTs decreased with increasing concentration, suggesting that intracellular SWCNTs accumulation is a saturable process. After refreshing culture medium, we found increasing SWCNTs in supernatant and decreasing intracellular SWCNTs over time, confirming the exocytosis occurred. Selective inhibition of endocytosis pathways showed that the internalization of SWCNTs involves several pathways, in the order of macropinocytosis> caveolae-mediated endocytosis> clathrin-dependent endocytosis. Intriguingly, clathrin-mediated endocytosis is relatively important for internalizing shorter SWCNTs. The dynamic processes of SWCNTs uptake and exocytosis and the mechanisms revealed by this study may render a better understanding on SWCNT toxicity and facilitate the design of CNT products with mitigated toxicity and desired functions. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39350]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Liang-Hong,Cui, Xuejing,Wan, Bin,et al. Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells[J]. SCIENTIFIC REPORTS,2017,7(0):-. |
| APA | Guo, Liang-Hong,Cui, Xuejing,Wan, Bin,Yang, Yu,&Ren, Xiaomin.(2017).Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells.SCIENTIFIC REPORTS,7(0),-. |
| MLA | Guo, Liang-Hong,et al."Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells".SCIENTIFIC REPORTS 7.0(2017):-. |
入库方式： OAI收割
来源：生态环境研究中心
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