Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals
文献类型：期刊论文
| 作者 | Yao, Xiaofang; Chen, Xiaoying; Zhang, Yinfeng; Li, Yuanyuan; Wang, Yao; Zheng, Zongming; Qin, Zhanfen; Zhang, Qingdong |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 52期号:0页码:314-324 |
| 关键词 | Thyroid Hormone Signaling Disruption Xenopus Laevis T3 Induction Morphological Endpoints Th-response Gene Expression |
| 英文摘要 | T3-induced Xenopus metamorphosis is an ideal model for detecting thyroid hormone (TH) signaling disruption of chemicals. To optimize the T3-induced Xenopus assay and improve its sensitivity and reproducibility, we intend to develop quantitatively morphological endpoints and choose appropriate concentrations and exposure durations for T3 induction. Xenopus laevis at stage 52 were exposed to series of concentrations of T3 (0.31-2.5 nmol/L) for 6 days. By comparing morphological changes induced by T3, we propose head area, mouth width, unilateral brain width/brain length, and hindlimb length/snout-vent length as quantitative parameters for characterizing T3-induced morphological changes, with body weight as a parameter for indicating integrated changes. By analyzing time-response curves, we found that following 4-day exposure, T3-induced grossly morphological changes displayed linear concentration-response curves, with moderate morphological changes resulting from 1.25 nmol/L T3 exposure. When using grossly morphological endpoints to detect TH signaling disruption, we propose 4 days as exposure duration of T3, with concentrations close to 1.25 nmol/L as induction concentrations. However, it is appropriate to examine morphological and molecular changes of the intestine on day 2 due to their early response to T3. The quantitative endpoints and T3 induction concentrations and durations we determined would improve the sensitivity and the reproducibility of the T3-induced Xenopus metamorphosis assay. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39375]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yao, Xiaofang,Chen, Xiaoying,Zhang, Yinfeng,et al. Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2017,52(0):314-324. |
| APA | Yao, Xiaofang.,Chen, Xiaoying.,Zhang, Yinfeng.,Li, Yuanyuan.,Wang, Yao.,...&Zhang, Qingdong.(2017).Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals.JOURNAL OF ENVIRONMENTAL SCIENCES,52(0),314-324. |
| MLA | Yao, Xiaofang,et al."Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals".JOURNAL OF ENVIRONMENTAL SCIENCES 52.0(2017):314-324. |
入库方式： OAI收割
来源：生态环境研究中心
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