Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA
文献类型：期刊论文
| 作者 | Han, Mingyong; Dong, Tianyi; Peng, Yanping; Chang, Lap Kam; Zhong, Ning; Liu, Fengyan; Zhang, Hanyu; Xu, Mengchen; Liu, Rutao; Tian, Xingsong |
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 8期号:31页码:50911-50920 |
| 关键词 | Pfda Proliferation Spla2-iia Tcf4 |
| 英文摘要 | The association of perfluorodecanoicacid (PFDA) with tumor promotion and associated effects is not clear. Given that PDFA is mostly consumed with food and drinking water, we evaluated the effects of PFDA on a gastric cell line. When added to cell cultures, PFDA significantly increased growth rate and colony forming ability compared with control treatment. We found that suppression of cell senescence, but not apoptosis or autophagy was associated with PFDA-induced promotion of cell amount. To determine the molecular mechanism that was involved, DNA microarray assays was used to analyze changes in gene expression in response to PFDA treatment. Data analysis demonstrated that the vascular endothelial growth factor signaling pathway had the lowest p-value, with sPLA2-IIA (pla2g2a) exhibits the most altered expression pattern within the pathway. Moreover, sPLA2-IIA and its transcription factor TCF4, known as a direct target and a binding partner of Wnt/beta-catenin signaling in gastric cells respectively, were the third and second most varied genes globally. Cells transfected with expression plasmids pENTER-tcf4 and pENTER-pla2g2a show reduced cell proliferation by more than 60% and 30% respectively. Knockdown with sPLA2-IIA siRNA provided additional evidence that sPLA2-IIA was a mediator of PFDA-induced cell senescence suppression. The results suggest for the first time that PFDA induced suppression of cell senescence through inhibition of sPLA2-IIA protein expression and might increased the proliferative capacity of an existing tumor. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39381]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Han, Mingyong,Dong, Tianyi,Peng, Yanping,et al. Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA[J]. ONCOTARGET,2017,8(31):50911-50920. |
| APA | Han, Mingyong.,Dong, Tianyi.,Peng, Yanping.,Chang, Lap Kam.,Zhong, Ning.,...&Guo, Liang-Hong.(2017).Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA.ONCOTARGET,8(31),50911-50920. |
| MLA | Han, Mingyong,et al."Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA".ONCOTARGET 8.31(2017):50911-50920. |
入库方式： OAI收割
来源：生态环境研究中心
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