Raman microspectroscopy of nucleus and cytoplasm for human colon cancer diagnosis
文献类型：期刊论文
| 作者 | Liu, Wenjing; Wang, Hongbo; Du, Jingjing; Jing, Chuanyong |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 97期号:0页码:70-74 |
| 关键词 | Subcellular Raman Actual Human Tissue Cls Model Pls-da Model |
| 英文摘要 | Subcellular Raman analysis is a promising clinic tool for cancer diagnosis, but constrained by the difficulty of deciphering subcellular spectra in actual human tissues. We report a label-free subcellular Raman analysis for use in cancer diagnosis that integrates subcellular signature spectra by subtracting cytoplasm from nucleus "spectra (Nuc.-Cyt.) with a partial least squares-discriminant analysis (PLS-DA) model. Raman mapping with the classical least-squares (CLS) model allowed direct visualization of the distribution of the cytoplasm and nucleus. The PLS-DA model was employed to evaluate the diagnostic performance of five types of spectral datasets, including non-selective, nucleus, cytoplasm, ratio of nucleus to cytoplasm (Nuc./Cyt.), and nucleus minus cytoplasm (Nuc.-Cyt.), resulting in diagnostic sensitivity of 88.3%, 84.0%, 98.4%, 84.5%, and 98.9%, respectively. Discriminating between normal and cancerous cells of actual human tissues through subcellular Raman markers is feasible, especially when using the nucleus-cytoplasm difference spectra. The subcellular Raman approach had good stability, and had excellent diagnostic performance for rectal as well as colon tissues. The insights gained from this study shed new light on the general applicability of subcellular Raman analysis in clinical trials. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39394]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Wenjing,Wang, Hongbo,Du, Jingjing,et al. Raman microspectroscopy of nucleus and cytoplasm for human colon cancer diagnosis[J]. BIOSENSORS & BIOELECTRONICS,2017,97(0):70-74. |
| APA | Liu, Wenjing,Wang, Hongbo,Du, Jingjing,&Jing, Chuanyong.(2017).Raman microspectroscopy of nucleus and cytoplasm for human colon cancer diagnosis.BIOSENSORS & BIOELECTRONICS,97(0),70-74. |
| MLA | Liu, Wenjing,et al."Raman microspectroscopy of nucleus and cytoplasm for human colon cancer diagnosis".BIOSENSORS & BIOELECTRONICS 97.0(2017):70-74. |
入库方式： OAI收割
来源：生态环境研究中心
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