Tetrachloro-1,4-benzoquinone induces apoptosis of mouse embryonic stem cells
文献类型：期刊论文
| 作者 | Li, Cuiping; Wang, Hailin; Wang, Fengbang |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 51期号:0页码:5-12 |
| 关键词 | Ten-eleven Translocation Tetrachloro-1 5-hydroxymethylcytosine 4-benzoquinone Early Apoptosis Late Apoptosis |
| 英文摘要 | Pentachlorophenol (PCP) is a widespread, persistent environmental contaminant, and it is enzymatically activated to form a reactive metabolite, tetrachloro-1,4-benzoquinone (TCBQ). To our knowledge, there is no information about TCBQ toxicity on embryonic stem cells. Here, we demonstrated that TCBQ induced significantly apoptosis of mouse embryonic stem cells in a concentration-dependent manner. We also showed that TCBQ elevated genomic 5-hydroxymethylcytosine (5hmC) by affecting ten-eleven translocation (Tet) dioxygenases in mouse embryonic stem cells. We further investigated whether Tet dioxygenases were implicated in TCBQ-induced apoptosis. By depleting all three dioxygenases (Tet1-3), we found that Tet dioxygenases slightly inhibited both early and late apoptosis induced by TCBQ at a low concentration (30 mu mol/L). Meanwhile, treated by TCBQ at higher concentrations (40 and 50 mu mol/L), the total percentage of apoptotic cells was not affected by Tet dioxygenases. However, Tet dioxygenases tended to arrest mouse ES cells to be at early apoptotic stage and to reduce the cells to enter later apoptotic stage. These results indicate that Tet dioxygenases play a role in shaping TCBQ-induced apoptosis in mouse embryonic stem cells. Our study provides new insights into the toxicology of PCP and its reactive metabolite TCBQ. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39431]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Cuiping,Wang, Hailin,Wang, Fengbang. Tetrachloro-1,4-benzoquinone induces apoptosis of mouse embryonic stem cells[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2017,51(0):5-12. |
| APA | Li, Cuiping,Wang, Hailin,&Wang, Fengbang.(2017).Tetrachloro-1,4-benzoquinone induces apoptosis of mouse embryonic stem cells.JOURNAL OF ENVIRONMENTAL SCIENCES,51(0),5-12. |
| MLA | Li, Cuiping,et al."Tetrachloro-1,4-benzoquinone induces apoptosis of mouse embryonic stem cells".JOURNAL OF ENVIRONMENTAL SCIENCES 51.0(2017):5-12. |
入库方式： OAI收割
来源：生态环境研究中心
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