中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation and characterization of a virulent bacteriophage infecting Acinetobacter johnsonii from activated sludge

文献类型:期刊论文

作者Qi, Rong; Yang, Min; Fan, Niansi
刊名RESEARCH IN MICROBIOLOGY
出版日期2017-06-01
卷号168期号:5页码:472-481
关键词Acinetobacter Johnsonii Bacteriophage Isolation Bacterial Infection Activated Sludge Wastewater
英文摘要A double-stranded DNA phage named AJO1, infecting Acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. Based on morphological taxonomy, AJO1, with an icosahedral head 55 +/- 2 nm in diameter and a non-contractile tail 8 +/- 2 nm in length, was classified as a member of the Podoviridae family. Bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multiplicity of infection of 10(-2); eclipse and burst size of 30 min and 51.2 PFU-infected cells(-1), respectively. It showed considerable infectivity under a neutral pH condition (pH 6.0-9.0) and relatively high temperature (55 degrees C). Whole-genome sequencing of AJO1 revealed a linearly permuted DNA (41 437 bp) carrying 54 putative open reading frames and 4 repeats. This is the first report of isolation of an A. johnsonii phage, whose bacteriophage distribution and population dynamics are not well known. The results of this study could contribute to subsequent research on the interaction between bacteriophages and their hosts during wastewater treatment. In addition, AJO1 may become a candidate for potential therapy against A. johnsonii infection in clinical applications, since this species is an opportunistic pathogen. (C) 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
源URL[http://ir.rcees.ac.cn/handle/311016/39521]  
专题生态环境研究中心_环境水质学国家重点实验室
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Qi, Rong,Yang, Min,Fan, Niansi. Isolation and characterization of a virulent bacteriophage infecting Acinetobacter johnsonii from activated sludge[J]. RESEARCH IN MICROBIOLOGY,2017,168(5):472-481.
APA Qi, Rong,Yang, Min,&Fan, Niansi.(2017).Isolation and characterization of a virulent bacteriophage infecting Acinetobacter johnsonii from activated sludge.RESEARCH IN MICROBIOLOGY,168(5),472-481.
MLA Qi, Rong,et al."Isolation and characterization of a virulent bacteriophage infecting Acinetobacter johnsonii from activated sludge".RESEARCH IN MICROBIOLOGY 168.5(2017):472-481.

入库方式: OAI收割

来源:生态环境研究中心

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