Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere
文献类型：期刊论文
| 作者 | Gu, Likun; Zhuang, Guoqiang; Jin, Bo; Hu, Qing; Bai, Zhihui; Wang, Huili; Zhang, Hongxun |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2010 |
| 卷号 | 22期号:1页码:134-141 |
| 关键词 | Fungicide Phyllosphere Microbial Community 16s Rdna Clone Library Pcr-dgge |
| 英文摘要 | Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the gamma-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application. |
| WOS记录号 | WOS:000273860800018 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21339]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Gu, Likun,Zhuang, Guoqiang,Jin, Bo,et al. Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2010,22(1):134-141. |
| APA | Gu, Likun.,Zhuang, Guoqiang.,Jin, Bo.,Hu, Qing.,Bai, Zhihui.,...&Zhang, Hongxun.(2010).Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,22(1),134-141. |
| MLA | Gu, Likun,et al."Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 22.1(2010):134-141. |
入库方式： OAI收割
来源：生态环境研究中心
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