Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor
文献类型：期刊论文
| 作者 | Zhao, Wenjuan; Hu, Qing; Li, Li; Wang, Yanjuan; Qi, Hongyan; Zhuang, Guoqiang |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2010 |
| 卷号 | 22期号:9页码:1469-1474 |
| 关键词 | Arsenic Reporter Gene Whole-cell Biosensor Yellow Fluorescent Protein (Phiyfp) |
| 英文摘要 | The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome of Escherichia coli DH5 alpha, and phiYFP was introduced into E. coli DH5 alpha as a reporter gene to construct an arsenic-resistant whole-cell biosensor (WCB-11) in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression of phiYFP. When strain WCB-11 was exposed to As(3+) and As(5+), the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field. |
| WOS记录号 | WOS:000282072900024 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21341]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao, Wenjuan,Hu, Qing,Li, Li,et al. Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2010,22(9):1469-1474. |
| APA | Zhao, Wenjuan,Hu, Qing,Li, Li,Wang, Yanjuan,Qi, Hongyan,&Zhuang, Guoqiang.(2010).Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,22(9),1469-1474. |
| MLA | Zhao, Wenjuan,et al."Construction of WCB-11: A novel phiYFP arsenic-resistant whole-cell biosensor".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 22.9(2010):1469-1474. |
入库方式： OAI收割
来源：生态环境研究中心
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