Functional display of fungal cellulases from Trichoderma reesei on phage M13
文献类型：期刊论文
| 作者 | Ma, Anzhou; Hu, Qing; Bai, Zhihui; Qu, Yinbo; Liu, Weifeng; Zhuang, Guoqiang |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2008-10 |
| 卷号 | 24期号:10页码:2003-2009 |
| 关键词 | Cellobiohydrolase i Endoglucanase i Phage-cellulase Phage Display Trichoderma Reesei |
| 英文摘要 | To test whether the phage display technology could be applied in cellulase engineering, phagemids harboring the genes encoding the mature forms of cellobiohydrolase I (CBH I) and endoglucanase I (EG I) from filamentous fungus Trichoderma reesei were constructed, respectively. CBH I and EG I fused to the phage coat protein encoded by the g3 gene were expressed and displayed on phage M13. The phage-bound cellulases retained their activities as determined by hydrolysis of the corresponding substrates, Also, their binding abilities to insoluble cellulose substrate were confirmed by an ELISA method. Overall, these results demonstrate that cellulases can be displayed on phage surface while maintaining their biological function, thus providing an alternative for directed evolution and high-throughput screening for improved cellulases. |
| WOS记录号 | WOS:000258658800001 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22350]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Ma, Anzhou,Hu, Qing,Bai, Zhihui,et al. Functional display of fungal cellulases from Trichoderma reesei on phage M13[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(10):2003-2009. |
| APA | Ma, Anzhou,Hu, Qing,Bai, Zhihui,Qu, Yinbo,Liu, Weifeng,&Zhuang, Guoqiang.(2008).Functional display of fungal cellulases from Trichoderma reesei on phage M13.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(10),2003-2009. |
| MLA | Ma, Anzhou,et al."Functional display of fungal cellulases from Trichoderma reesei on phage M13".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.10(2008):2003-2009. |
入库方式： OAI收割
来源：生态环境研究中心
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