Purification and characterization of levoglucosan kinase from Lipomyces starkeyi YZ-215
文献类型：期刊论文
| 作者 | Ning, Jiying; Yu, Zhisheng; Xie, Huijun; Zhang, Hongxun; Zhuang, Guoqiang; Bai, Zhihui; Yang, Shengmei; Jiang, Yongmin |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2008-01 |
| 卷号 | 24期号:1页码:15-22 |
| 关键词 | Levoglucosan Kinase 1 Purification 6-anhydro-beta-d-glucopyranose Lipomyces Starkeyi Yz-215 Maldi-tof Ms Esi-q-tof Ms/ms |
| 英文摘要 | A protein named as levoglucosan kinase (EC 2.7.-.-)was purified to homogeneity from a wild isolated strain of Lipomyces starkeyi YZ-215. The protein was purified approximately 30-fold by conventional ammonium sulphate fractionation followed by Resource Q chromatography and two steps of Superdex 200 chromatography, and its physical and kinetic properties were investigated. The purified enzyme showed a molecular weight of 48 kDa by SDS-PAGE and 47.7 kDa by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), respectively. The enzyme was stable at pH 7-10 and showed maximum activity at 30 degrees C and pH 9.0. Kinetic constants (apparent K-m values) for levoglucosan and ATP were 68.6 +/- 13.7 mM and 0.68 +/- 0.06 mM, respectively. After in-gel digestion by trypsin, three peptides were sequenced and analyzed by electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF MS/MS). Data of the amino acid sequences indicated that this protein might be a novel kinase. The purification of levoglucosan kinase from L. starkeyi YZ-215 represented a fundamental step to provide insights into the efficient utilization of cellulosic pyrolysate by bioconversion. |
| WOS记录号 | WOS:000251887400003 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22352]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Ning, Jiying,Yu, Zhisheng,Xie, Huijun,et al. Purification and characterization of levoglucosan kinase from Lipomyces starkeyi YZ-215[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(1):15-22. |
| APA | Ning, Jiying.,Yu, Zhisheng.,Xie, Huijun.,Zhang, Hongxun.,Zhuang, Guoqiang.,...&Jiang, Yongmin.(2008).Purification and characterization of levoglucosan kinase from Lipomyces starkeyi YZ-215.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(1),15-22. |
| MLA | Ning, Jiying,et al."Purification and characterization of levoglucosan kinase from Lipomyces starkeyi YZ-215".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.1(2008):15-22. |
入库方式： OAI收割
来源：生态环境研究中心
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