The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I
文献类型：期刊论文
| 作者 | Ma, Anzhou; Hu, Qing; Qu, Yinbo; Bai, Zhihui; Liu, Weifeng; Zhuang, Guoqiang |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2008-06-05 |
| 卷号 | 42期号:7页码:543-547 |
| 关键词 | Cellobiohydrolase i Hydrolysis Irreversible Adsorption Solid-liquid Interface Rate Retardation |
| 英文摘要 | Protein adsorption onto solid substrates usually takes place in an irreversible fashion and this irreversible adsorption also occurs in some enzymatic reactions. In this work the adsorption behavior of intact beta-1, 4-glucan-cellobiohydrolase (CBH I) from Trichoderma reesei onto microcrystalline cellulose was monitored by surface plasmon resonance and UV-spectral method. It was found that there existed reversible binding and irreversible binding of CBH I during its interaction with cellulose substrate. To evaluate the influence of adsorption on cellulose enzymatic hydrolysis, the reaction dynamics on pure cellulose were determined. A plot of the hydrolysis rate against the surface density of irreversibly adsorbed CBH I, revealed an inverse relationship in which an apparent decrease in the hydrolysis rate was observed with increasing surface density. Taken together, results presented here should be useful for modifying the binding characteristics of CBH I and making them more effective in cellulose hydrolysis. (c) 2008 Elsevier Inc. All rights reserved. |
| WOS记录号 | WOS:000256640400002 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22357]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Ma, Anzhou,Hu, Qing,Qu, Yinbo,et al. The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I[J]. ENZYME AND MICROBIAL TECHNOLOGY,2008,42(7):543-547. |
| APA | Ma, Anzhou,Hu, Qing,Qu, Yinbo,Bai, Zhihui,Liu, Weifeng,&Zhuang, Guoqiang.(2008).The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I.ENZYME AND MICROBIAL TECHNOLOGY,42(7),543-547. |
| MLA | Ma, Anzhou,et al."The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I".ENZYME AND MICROBIAL TECHNOLOGY 42.7(2008):543-547. |
入库方式： OAI收割
来源：生态环境研究中心
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